Interferon gamma production during bovine respiratory syncytial virus (BRSV) infection is diminished in calves vaccinated with formalin-inactivated BRSV

Amelia R. Woolums, Randall S. Singer, Gabrielle A. Boyle, Laurel J Gershwin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Formalin-inactivated respiratory syncytial virus (FI-RSV) vaccination has been associated with severe disease in humans. Research in mice suggests that FI-RSV may prime for decreased interferon gamma (IFN-γ) production at subsequent infection. Interferon-γ production by peripheral blood mononuclear cells (PBMC) was measured following challenge of calves vaccinated with FI-BRSV to determine whether a similar mechanism is operative in a host naturally susceptible to RSV. Eight-week old male Holstein calves were administered FI-BRSV and mock challenge (V/M, n = 6); mock vaccination and BRSV challenge (M/C, n = 6) or FI-BRSV and BRSV challenge (V/C, n = 7). Vaccine was administered twice at a 2-week interval; challenge followed one month later. On days 0, 5 and 10 postchallenge (PC), PBMC were stimulated in vitro for 24 h with live BRSV, concanavalin A (positive control) or spent media (negative control). Supernatants were assayed for IFN-γ using ELISA. Interferon-γ production by BRSV-stimulated PBMC was increased in M/C and V/C calves as compared to V/M calves on day 5 PC (p < 0.015); and increased in M/C calves compared to V/C and V/M Calves on day 10 PC (p < 0.015). Over time postchallenge, a significant increase in IFN-γ production by BRSV-stimulated PBMC was seen in M/C calves (p < 0.025) but not in V/C calves. FI-BRSV vaccination of calves led to diminished IFN-γ production postchallenge. Decreased IFN-γ production may have contributed to impaired viral clearance and enhanced disease in FI-BRSV vaccinated calves.

Original languageEnglish (US)
Pages (from-to)1293-1297
Number of pages5
JournalVaccine
Volume17
Issue number11-12
DOIs
StatePublished - Mar 1999

Fingerprint

Bovine respiratory syncytial virus
Respiratory Syncytial Virus Infections
interferon-gamma
formalin
Formaldehyde
Interferon-gamma
calves
infection
mononuclear leukocytes
Blood Cells
Vaccination
Respiratory Syncytial Viruses
vaccination
interferons
Interferons
viruses
concanavalin A
Concanavalin A
human diseases
Holstein

Keywords

  • BRSV
  • Formalin-inactivated RSV
  • Interferon gamma

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Interferon gamma production during bovine respiratory syncytial virus (BRSV) infection is diminished in calves vaccinated with formalin-inactivated BRSV. / Woolums, Amelia R.; Singer, Randall S.; Boyle, Gabrielle A.; Gershwin, Laurel J.

In: Vaccine, Vol. 17, No. 11-12, 03.1999, p. 1293-1297.

Research output: Contribution to journalArticle

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abstract = "Formalin-inactivated respiratory syncytial virus (FI-RSV) vaccination has been associated with severe disease in humans. Research in mice suggests that FI-RSV may prime for decreased interferon gamma (IFN-γ) production at subsequent infection. Interferon-γ production by peripheral blood mononuclear cells (PBMC) was measured following challenge of calves vaccinated with FI-BRSV to determine whether a similar mechanism is operative in a host naturally susceptible to RSV. Eight-week old male Holstein calves were administered FI-BRSV and mock challenge (V/M, n = 6); mock vaccination and BRSV challenge (M/C, n = 6) or FI-BRSV and BRSV challenge (V/C, n = 7). Vaccine was administered twice at a 2-week interval; challenge followed one month later. On days 0, 5 and 10 postchallenge (PC), PBMC were stimulated in vitro for 24 h with live BRSV, concanavalin A (positive control) or spent media (negative control). Supernatants were assayed for IFN-γ using ELISA. Interferon-γ production by BRSV-stimulated PBMC was increased in M/C and V/C calves as compared to V/M calves on day 5 PC (p < 0.015); and increased in M/C calves compared to V/C and V/M Calves on day 10 PC (p < 0.015). Over time postchallenge, a significant increase in IFN-γ production by BRSV-stimulated PBMC was seen in M/C calves (p < 0.025) but not in V/C calves. FI-BRSV vaccination of calves led to diminished IFN-γ production postchallenge. Decreased IFN-γ production may have contributed to impaired viral clearance and enhanced disease in FI-BRSV vaccinated calves.",
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