Interferon γ Induces Retrograde Dendritic Retraction and Inhibits Synapse Formation

In Jung Kim, Hiroko Nagasawa Beck, Pamela J Lein, Dennis Higgins

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The expression of interferon γ (IFNγ) increases after neural injury, and it is sustained in chronic inflammatory conditions such as multiple sclerosis and infection with human immunodeficiency virus. To understand how exposure to this proinflammatory cytokine might affect neural function, we examined its effects on cultures of neurons derived from the central and peripheral nervous systems. IFNγ inhibits initial dendritic outgrowth in cultures of embryonic rat sympathetic and hippocampal neurons, and this inhibitory effect on process growth is associated with a decrease in the rate of synapse formation. In addition, in older cultures of sympathetic neurons, IFNγ also selectively induces retraction of existing dendrites, ultimately leading to an 88% decrease in the size of the arbor. Dendritic retraction induced by IFNγ represents a specific cellular response because it occurs without affecting axonal outgrowth or cell survival, and it is not observed with tumor necrosis factor α or other inflammatory cytokines. IFN-γ-induced dendritic retraction is associated with the phosphorylation and nuclear translocation of signal transducer and activator of transcription 1 (STAT1), and expression of a dominant-negative STAT1 construct attenuates the inhibitory effect of IFNγ. Moreover, retrograde dendritic retraction is observed when distal axons are selectively exposed to IFNγ. These data imply that IFNγ-mediated STAT1 activation induces both dendritic atrophy and synaptic loss and that this occurs both at the sites of IFNγ release and at remote loci. Regressive actions of IFNγ on dendrites may contribute to the neuropathology of inflammatory diseases.

Original languageEnglish (US)
Pages (from-to)4530-4539
Number of pages10
JournalJournal of Neuroscience
Volume22
Issue number11
StatePublished - Jun 1 2002
Externally publishedYes

Fingerprint

Synapses
Interferons
STAT1 Transcription Factor
Dendrites
Neurons
Cytokines
Peripheral Nervous System
Transcriptional Activation
Multiple Sclerosis
Atrophy
Axons
Cell Survival
Central Nervous System
Tumor Necrosis Factor-alpha
Phosphorylation
HIV
Wounds and Injuries
Growth
Infection

Keywords

  • Bone morphogenetic protein-7
  • Dendrites
  • Hippocampal neurons
  • Interferon γ
  • Retrograde transport
  • STAT1
  • Sympathetic neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Interferon γ Induces Retrograde Dendritic Retraction and Inhibits Synapse Formation. / Kim, In Jung; Beck, Hiroko Nagasawa; Lein, Pamela J; Higgins, Dennis.

In: Journal of Neuroscience, Vol. 22, No. 11, 01.06.2002, p. 4530-4539.

Research output: Contribution to journalArticle

Kim, In Jung ; Beck, Hiroko Nagasawa ; Lein, Pamela J ; Higgins, Dennis. / Interferon γ Induces Retrograde Dendritic Retraction and Inhibits Synapse Formation. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 11. pp. 4530-4539.
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