Interferon-γ enhances susceptibility of cervical cancer cells to lysis by tumor-specific cytotoxic T cells

Daron Street, Andreas M. Kaufmann, Andrew T M Vaughan, Susan G. Fisher, Michael Hunter, Carola Schreckenberger, Ronald K. Potkul, Lutz Gissmann, Liang Qiao

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Recently we have demonstrated that tumor-specific cytotoxic T lymphocytes (Culs) can be activated by cervical carcinoma cells expressing the costimulatory molecule CD80, which may be used as a therapeutic vaccine for patients with cervical cancer. For activated Culs to be effective, appropriate amounts of MHC class I expression are required on target tumor cells. In this study, we found that some cervical carcinoma cells expressed only low levels of MHC class 1 and adhesion molecules such as CD54. We further demonstrated that tumor cells (CaSki and SiHa) expressing low levels of MHC class 1 were more resistant to lysis by specific Culs than tumor cells (HeLa) expressing high levels of MHC class I. Treatment of CaSki or SiHa cells with interferon-γ resulted in n increased expression of MHC class I, MHC class II, and CD54. Expression of CD58 and CD80 was not up-regulated or induced. Treatment of the tumor cells with interferon-γ significantly enhanced the lysis of the tumor cells by specific Culs which had been activated by the respective CD80-expressing tumor cells. The enhancement of cytolysis could be blocked by monoclonal antibodies to MHC class I and CD54, but not by that to MHC class II. Furthermore, we found that interferon-γ induced apoptosis in cervical carcinoma cells but not in tumor-specific Culs.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalGynecologic Oncology
Volume65
Issue number2
DOIs
StatePublished - May 1997
Externally publishedYes

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

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    Street, D., Kaufmann, A. M., Vaughan, A. T. M., Fisher, S. G., Hunter, M., Schreckenberger, C., Potkul, R. K., Gissmann, L., & Qiao, L. (1997). Interferon-γ enhances susceptibility of cervical cancer cells to lysis by tumor-specific cytotoxic T cells. Gynecologic Oncology, 65(2), 265-272. https://doi.org/10.1006/gyno.1997.4667