Interactions of the cytoplasmic domains of human and simian retroviral transmembrane proteins with components of the clathrin adaptor complexes modulate intracellular and cell surface expression of envelope glycoproteins

Clarisse Berlioz-Torrent, Barbara Shacklett, Lars Erdtmann, Lelia Delamarre, Isabelle Bouchaert, Pierre Sonigo, Marie Christine Dokhelar, Richard Benarous

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

The cytoplasmic domains of the transmembrane (TM) envelope proteins (TM- CDs) of most retroviruses have a Tyr-based motif, YXXφ, in their membrane- proximal regions. This signal is involved in the trafficking and endocytosis of membrane receptors via clathrin-associated AP-1 and AP-2 adaptor complexes. We have used CD8-TM-CD chimeras to investigate the role of the Tyr-based motif of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus (SIV), and human T-leukemia virus type 1 (HTLV-1) TM- CDs in the cell surface expression of the envelope glycoprotein. Flow cytometry and confocal microscopy studies showed that this motif is a major determinant of the cell surface expression of the CD8-HTLV chimera. The YXXφ motif also plays a key role in subcellular distribution of the envelope of lentiviruses HIV-1 and SIV. However, these viruses, which encode TM proteins with a long cytoplasmic domain, have additional determinants distal to the YXXφ motif that participate in regulating cell surface expression. We have also used the yeast two-hybrid system and in vitro binding assays to demonstrate that all three retroviral YXXφ motifs interact with the μ1 and μ2 subunits of AP complexes and that the C-terminal regions of HIV-1 and SIV TM proteins interact with the β2 adaptin subunit. The TM-CDs of HTLV-1, HIV- 1, and SIV also interact with the whole AP complexes. These results clearly demonstrate that the cell surface expression of retroviral envelope glycoproteins is governed by interactions with adaptor complexes. The YXXφ- based signal is the major determinant of this interaction for the HTLV-1 TM, which contains a short cytoplasmic domain, whereas the lentiviruses HIV-1 and SIV have additional determinants distal to this signal that are also involved.

Original languageEnglish (US)
Pages (from-to)1350-1361
Number of pages12
JournalJournal of Virology
Volume73
Issue number2
StatePublished - 1999
Externally publishedYes

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Vesicular Transport Adaptor Proteins
Simian immunodeficiency virus
clathrin
transmembrane proteins
Human immunodeficiency virus 1
Simian Immunodeficiency Virus
HIV-1
glycoproteins
Glycoproteins
leukemia
Viruses
viruses
Lentivirus
Leukemia
chimerism
Proteins
cells
Retroviridae
two hybrid system techniques
Clathrin

ASJC Scopus subject areas

  • Immunology

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Interactions of the cytoplasmic domains of human and simian retroviral transmembrane proteins with components of the clathrin adaptor complexes modulate intracellular and cell surface expression of envelope glycoproteins. / Berlioz-Torrent, Clarisse; Shacklett, Barbara; Erdtmann, Lars; Delamarre, Lelia; Bouchaert, Isabelle; Sonigo, Pierre; Dokhelar, Marie Christine; Benarous, Richard.

In: Journal of Virology, Vol. 73, No. 2, 1999, p. 1350-1361.

Research output: Contribution to journalArticle

Berlioz-Torrent, Clarisse ; Shacklett, Barbara ; Erdtmann, Lars ; Delamarre, Lelia ; Bouchaert, Isabelle ; Sonigo, Pierre ; Dokhelar, Marie Christine ; Benarous, Richard. / Interactions of the cytoplasmic domains of human and simian retroviral transmembrane proteins with components of the clathrin adaptor complexes modulate intracellular and cell surface expression of envelope glycoproteins. In: Journal of Virology. 1999 ; Vol. 73, No. 2. pp. 1350-1361.
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AU - Erdtmann, Lars

AU - Delamarre, Lelia

AU - Bouchaert, Isabelle

AU - Sonigo, Pierre

AU - Dokhelar, Marie Christine

AU - Benarous, Richard

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