Interactions of Recombinant and Wild-Type Baculoviruses with Classical Insecticides and Pyrethroid-Resistant Tobacco Budworm (Lepidoptera: Noctuidae)

B. F. Mccutchen, K. Hoover, H. K. Preisler, M. D. Betana, R. Herrmann, J. L. Robertson, B. D. Hammock

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

In tests with neonate Heliothis virescens (F.), we characterized interactions of all combinations of a recombinant Autographa californica (Speyer) nuclear polyhedrosis virus (AcAaIT) that expresses an insect-selective neurotoxin (AaIT) and wild-type AcNPV when combined with low concentrations of several conventional insecticides. All combinations of the recombinant virus AcAaIT and insecticides showed a positive interaction (decrease in the median lethal time (LT50) compared with the LT50 for either component alone). A type II pyrethroid (cypermethrin, which modifies currents of sodium channels) and a carbamate (methomyl, an inhibitor of acetylcholinesterase) were synergistic in combination with AcAaIT. Other insecticides also showed a positive interaction when tested in combination with the recombinant virus, but joint activity was slightly antagonistic (i.e., less than predicted activity when combined) with wild-type AcNPV. We also characterized the effectiveness of AcAaIT against pyrethroid-resistant H. virescens larvae. Our results show that a resistant strain of H. virescens is more sensitive to the recombinant virus compared with a susceptible strain. Results of these studies should be useful in planning of future field trials to increase the effectiveness of nuclear polyhedrosis viruses and to manage resistance to pyrethroids and other insecticides.

Original languageEnglish (US)
Pages (from-to)1170-1180
Number of pages11
JournalJournal of Economic Entomology
Volume90
Issue number5
StatePublished - Oct 1997

Keywords

  • Baculovirus
  • Heliothis virescens
  • Insecticide
  • Nuclear polyhedrosis virus
  • Pyrethroid
  • Recombinant microorganism

ASJC Scopus subject areas

  • Insect Science

Fingerprint Dive into the research topics of 'Interactions of Recombinant and Wild-Type Baculoviruses with Classical Insecticides and Pyrethroid-Resistant Tobacco Budworm (Lepidoptera: Noctuidae)'. Together they form a unique fingerprint.

  • Cite this

    Mccutchen, B. F., Hoover, K., Preisler, H. K., Betana, M. D., Herrmann, R., Robertson, J. L., & Hammock, B. D. (1997). Interactions of Recombinant and Wild-Type Baculoviruses with Classical Insecticides and Pyrethroid-Resistant Tobacco Budworm (Lepidoptera: Noctuidae). Journal of Economic Entomology, 90(5), 1170-1180.