Interactions of peroxynitrite with human plasma and its constituents: Oxidative damage and antioxidant depletion

A. Van der Vliet, D. Smith, C. A. O'Neill, H. Kaur, V. Darley-Usmar, Carroll E Cross, B. Halliwell

Research output: Contribution to journalArticle

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Abstract

Endothelial cells and activated phagocytes produce both nitric oxide ((̇)No) and superoxide (O2(̇-)), which react to form peroxynitrite. Peroxynitrite has been suggested to be directly cytotoxic and also to decompose into other toxic species. In order to understand the consequences of peroxynitrite generation in vivo, we examined its reaction with human blood plasma. Peroxynitrite decreased the total peroxyl-trapping capacity of plasma. In terms of specific antioxidants, addition of peroxynitrite to plasma leads to rapid oxidation of ascorbic acid, uric acid and plasma SH groups. The oxidation of plasma SH groups was enhanced in dialysed plasma and returned to control levels by the addition of physiological levels of bicarbonate. Evidence was found for formation of nitro-adducts to aromatic side chains in plasma proteins by peroxynitrite. Peroxynitrite also leads to depletion of ubiquinol and formation of traces of lipid hydroperoxides in plasma, although α-tocopherol levels were only slightly decreased. Peroxynitrite formation in human body fluids is likely to cause antioxidant depletion and oxidative damage.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalBiochemical Journal
Volume303
Issue number1
StatePublished - 1994

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Plasma (human)
Peroxynitrous Acid
Antioxidants
Plasmas
Oxidation
Tocopherols
Lipid Peroxides
Poisons
Body fluids
Level control
Endothelial cells
Body Fluids
Phagocytes
Bicarbonates
Uric Acid
Human Body
Superoxides
Ascorbic Acid
Blood Proteins
Nitric Oxide

ASJC Scopus subject areas

  • Biochemistry

Cite this

Van der Vliet, A., Smith, D., O'Neill, C. A., Kaur, H., Darley-Usmar, V., Cross, C. E., & Halliwell, B. (1994). Interactions of peroxynitrite with human plasma and its constituents: Oxidative damage and antioxidant depletion. Biochemical Journal, 303(1), 295-301.

Interactions of peroxynitrite with human plasma and its constituents : Oxidative damage and antioxidant depletion. / Van der Vliet, A.; Smith, D.; O'Neill, C. A.; Kaur, H.; Darley-Usmar, V.; Cross, Carroll E; Halliwell, B.

In: Biochemical Journal, Vol. 303, No. 1, 1994, p. 295-301.

Research output: Contribution to journalArticle

Van der Vliet, A, Smith, D, O'Neill, CA, Kaur, H, Darley-Usmar, V, Cross, CE & Halliwell, B 1994, 'Interactions of peroxynitrite with human plasma and its constituents: Oxidative damage and antioxidant depletion', Biochemical Journal, vol. 303, no. 1, pp. 295-301.
Van der Vliet, A. ; Smith, D. ; O'Neill, C. A. ; Kaur, H. ; Darley-Usmar, V. ; Cross, Carroll E ; Halliwell, B. / Interactions of peroxynitrite with human plasma and its constituents : Oxidative damage and antioxidant depletion. In: Biochemical Journal. 1994 ; Vol. 303, No. 1. pp. 295-301.
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