Abstract
The functional characteristics of neutrophils are exceedingly sensitive to physiological conditions as well as the details of isolation. Exposure to lipopolysaccharide (LPS) or even contamination of the isolating media with traces of LPS is known to play an important role in regulating cell function and expression of receptors. Because of the suspected role of CD14 as a receptor for LPS, we used anti-CD14 monoclonal antibodies both to identify CD14 in the cell surface of polymorphonuclear leukocytes and to inhibit functional changes elicited by LPS. Cytometric techniques were used to investigate the regulation of CD14 and CR3 on the neutrophil cell surface in whole blood to minimize any effects of isolation. In whole blood neutrophils express low levels of formyl peptide receptor, CD14, and CR3, which increase substantially in response to formyl peptide and LPS. The increases in CR3 and CD14 occurred in parallel and were independent of protein synthesis and tumor necrosis factor (TNF) production. The increase in CR3 was inhibited by antibodies MY4, 3C10, and 28C5 against CD14. These findings are consistent with the notion that in blood the observed receptor up-regulation is in direct response to the action of LPS on neutrophils through CD14 and does not require products from macrophages such as TNF or the production of C5a from the plasma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 518-524 |
| Number of pages | 7 |
| Journal | Journal of Leukocyte Biology |
| Volume | 53 |
| Issue number | 5 |
| State | Published - 1993 |
| Externally published | Yes |
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Keywords
- CD14
- CR3
- gram-negative sepsis
- lipopolysaccharide
- lipopolysaccharide-binding protein
- priming
ASJC Scopus subject areas
- Cell Biology
Cite this
Interactions of lipopolysaccharide with neutrophils in blood via CD14. / Weingarten, R.; Sklar, L. A.; Mathison, J. C.; Omidi, S.; Ainsworth, T.; Simon, S.; Ulevitch, R. J.; Tobias, P. S.
In: Journal of Leukocyte Biology, Vol. 53, No. 5, 1993, p. 518-524.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interactions of lipopolysaccharide with neutrophils in blood via CD14
AU - Weingarten, R.
AU - Sklar, L. A.
AU - Mathison, J. C.
AU - Omidi, S.
AU - Ainsworth, T.
AU - Simon, S.
AU - Ulevitch, R. J.
AU - Tobias, P. S.
PY - 1993
Y1 - 1993
N2 - The functional characteristics of neutrophils are exceedingly sensitive to physiological conditions as well as the details of isolation. Exposure to lipopolysaccharide (LPS) or even contamination of the isolating media with traces of LPS is known to play an important role in regulating cell function and expression of receptors. Because of the suspected role of CD14 as a receptor for LPS, we used anti-CD14 monoclonal antibodies both to identify CD14 in the cell surface of polymorphonuclear leukocytes and to inhibit functional changes elicited by LPS. Cytometric techniques were used to investigate the regulation of CD14 and CR3 on the neutrophil cell surface in whole blood to minimize any effects of isolation. In whole blood neutrophils express low levels of formyl peptide receptor, CD14, and CR3, which increase substantially in response to formyl peptide and LPS. The increases in CR3 and CD14 occurred in parallel and were independent of protein synthesis and tumor necrosis factor (TNF) production. The increase in CR3 was inhibited by antibodies MY4, 3C10, and 28C5 against CD14. These findings are consistent with the notion that in blood the observed receptor up-regulation is in direct response to the action of LPS on neutrophils through CD14 and does not require products from macrophages such as TNF or the production of C5a from the plasma.
AB - The functional characteristics of neutrophils are exceedingly sensitive to physiological conditions as well as the details of isolation. Exposure to lipopolysaccharide (LPS) or even contamination of the isolating media with traces of LPS is known to play an important role in regulating cell function and expression of receptors. Because of the suspected role of CD14 as a receptor for LPS, we used anti-CD14 monoclonal antibodies both to identify CD14 in the cell surface of polymorphonuclear leukocytes and to inhibit functional changes elicited by LPS. Cytometric techniques were used to investigate the regulation of CD14 and CR3 on the neutrophil cell surface in whole blood to minimize any effects of isolation. In whole blood neutrophils express low levels of formyl peptide receptor, CD14, and CR3, which increase substantially in response to formyl peptide and LPS. The increases in CR3 and CD14 occurred in parallel and were independent of protein synthesis and tumor necrosis factor (TNF) production. The increase in CR3 was inhibited by antibodies MY4, 3C10, and 28C5 against CD14. These findings are consistent with the notion that in blood the observed receptor up-regulation is in direct response to the action of LPS on neutrophils through CD14 and does not require products from macrophages such as TNF or the production of C5a from the plasma.
KW - CD14
KW - CR3
KW - gram-negative sepsis
KW - lipopolysaccharide
KW - lipopolysaccharide-binding protein
KW - priming
UR - http://www.scopus.com/inward/record.url?scp=0027196851&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027196851&partnerID=8YFLogxK
M3 - Article
C2 - 7684764
AN - SCOPUS:0027196851
VL - 53
SP - 518
EP - 524
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 5
ER -