Interactions of ascorbic acid supplementation and bleomycin instillation on murine lung connective tissue metabolism

M. A. Dubick, Jerold A Last, Carroll E Cross, R. B. Rucker

Research output: Contribution to journalArticle

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Abstract

The effects of ascorbic acid supplementation on the pulmonary toxicity induced by bleomycin were examined. Swiss-Webster mice were fed an ascorbate-free diet supplemented with ascorbic acid at 0%, 0.2%, or 1.0% of the diet for 2 weeks. Bleomycin (0.15 units) was instilled intratracheally and the mice were killed 1 week later. Bleomycin caused pulmonary inflammation and edema as noted by the increases in lung wet weight and lung wet-weight-to-dry-weight ratios. The activity of prolyl hydroxylase was increased 1.4-fold to 1.6-fold in response to bleomycin, but only minor increases were observed in the collagen and elastin content of the lung. Prior dietary ascorbic acid supplementation did not reverse the effects induced by bleomycin. Interestingly, each dietary level of supplemental ascorbic acid resulted in a slight increase in the elastin and collagen content of the lung in comparison with lungs from mice consuming no ascorbic acid in their diet. The data suggest that high levels of ascorbic acid supplementation may aggravate the response to bleomycin.

Original languageEnglish (US)
Pages (from-to)105-115
Number of pages11
JournalDrug-Nutrient Interactions
Volume2
Issue number2
StatePublished - 1983

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Bleomycin
Connective Tissue
Ascorbic Acid
Lung
Pulmonary Edema
Elastin
Diet
Weights and Measures
Collagen
Prolyl Hydroxylases
Pneumonia

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology

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Interactions of ascorbic acid supplementation and bleomycin instillation on murine lung connective tissue metabolism. / Dubick, M. A.; Last, Jerold A; Cross, Carroll E; Rucker, R. B.

In: Drug-Nutrient Interactions, Vol. 2, No. 2, 1983, p. 105-115.

Research output: Contribution to journalArticle

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AB - The effects of ascorbic acid supplementation on the pulmonary toxicity induced by bleomycin were examined. Swiss-Webster mice were fed an ascorbate-free diet supplemented with ascorbic acid at 0%, 0.2%, or 1.0% of the diet for 2 weeks. Bleomycin (0.15 units) was instilled intratracheally and the mice were killed 1 week later. Bleomycin caused pulmonary inflammation and edema as noted by the increases in lung wet weight and lung wet-weight-to-dry-weight ratios. The activity of prolyl hydroxylase was increased 1.4-fold to 1.6-fold in response to bleomycin, but only minor increases were observed in the collagen and elastin content of the lung. Prior dietary ascorbic acid supplementation did not reverse the effects induced by bleomycin. Interestingly, each dietary level of supplemental ascorbic acid resulted in a slight increase in the elastin and collagen content of the lung in comparison with lungs from mice consuming no ascorbic acid in their diet. The data suggest that high levels of ascorbic acid supplementation may aggravate the response to bleomycin.

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