Interactions of a monoclonal antibody to the insulin receptor with receptors for insulin-like growth factors

R. A. Roth, B. Maddux, K. Y. Wong, Dennis M Styne, G. Van Vliet, R. E. Humbel, I. D. Goldfine

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

A monoclonal antibody to the human insulin receptor was tested for its ability to inhibit the binding of 125I-insulin-like growth factor I (IGF-I) and 125I-insulin-like growth factor II (IGF-II) to their receptors in human placenta membranes and cultured human IM-9 lymphocytes. In both placenta membranes and IM-9 cells, the antibody progressively inhibited the binding of 125I-IGF-I to its receptor with a potency that was 300-fold less than its ability to inhibit the binding of 125I-insulin to its own receptor. In contrast, in human placenta membranes, this antibody inhibited the binding of 125I-IGF-II to its receptor only slightly. These studies indicate, therefore, that this monoclonal antibody binds preferentially to the insulin receptor but also crossreacts to a lesser extent with the IGF-I receptor.

Original languageEnglish (US)
Pages (from-to)1865-1867
Number of pages3
JournalEndocrinology
Volume112
Issue number5
StatePublished - 1983

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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    Roth, R. A., Maddux, B., Wong, K. Y., Styne, D. M., Van Vliet, G., Humbel, R. E., & Goldfine, I. D. (1983). Interactions of a monoclonal antibody to the insulin receptor with receptors for insulin-like growth factors. Endocrinology, 112(5), 1865-1867.