Abstract
The interaction of Rhodococcus equi with alveolar macrophages from adult horses, foals experimentally exposed to R. equi (sensitized foals) and non-exposed foals was studied using in vitro bactericidal assays, cytochemical staining and transmission electron microscopy. It was demonstrated that R. equi is a facultative intracellular parasite, able to survive and multiply within the alveolar macrophages of the host by interfering with phagosome-lysosome fusion. Opsonization of R. equi with antibody against capsular components was associated with increased phagosome-lysosome fusion and significantly enhanced (P<0.05) killing of the organism by alveolar macrophages from non-exposed foals. Macrophages from non-exposed foals were able to ingest the non-opsonized organism, but unable to kill > 65% of the infective dose by 6 h post-exposure. Alveolar macrophages from sensitized foals behaved as adult macrophages, able to kill > 95% of the infective dose by 6 h. Lymphocyte factors, derived by in vitro incubation of sensitized peripheral blood lymphocytes with R. equi surface antigens, enhanced macrophage bactericidal activity. Macrophages from non-exposed foals incubated in the presence of the lymphocyte factors had a 50% increase in killing of R. equi, while sensitized macrophages incubated with lymphocyte factors had a > 100% increase in killing capacity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 307-320 |
| Number of pages | 14 |
| Journal | Veterinary Microbiology |
| Volume | 14 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1987 |
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ASJC Scopus subject areas
- Microbiology
- veterinary(all)
Cite this
Interaction of Rhodococcus equi with phagocytic cells from R. equi-exposed and non-exposed foals. / Hietala, S. K.; Ardans, Alex.
In: Veterinary Microbiology, Vol. 14, No. 3, 01.01.1987, p. 307-320.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interaction of Rhodococcus equi with phagocytic cells from R. equi-exposed and non-exposed foals
AU - Hietala, S. K.
AU - Ardans, Alex
PY - 1987/1/1
Y1 - 1987/1/1
N2 - The interaction of Rhodococcus equi with alveolar macrophages from adult horses, foals experimentally exposed to R. equi (sensitized foals) and non-exposed foals was studied using in vitro bactericidal assays, cytochemical staining and transmission electron microscopy. It was demonstrated that R. equi is a facultative intracellular parasite, able to survive and multiply within the alveolar macrophages of the host by interfering with phagosome-lysosome fusion. Opsonization of R. equi with antibody against capsular components was associated with increased phagosome-lysosome fusion and significantly enhanced (P<0.05) killing of the organism by alveolar macrophages from non-exposed foals. Macrophages from non-exposed foals were able to ingest the non-opsonized organism, but unable to kill > 65% of the infective dose by 6 h post-exposure. Alveolar macrophages from sensitized foals behaved as adult macrophages, able to kill > 95% of the infective dose by 6 h. Lymphocyte factors, derived by in vitro incubation of sensitized peripheral blood lymphocytes with R. equi surface antigens, enhanced macrophage bactericidal activity. Macrophages from non-exposed foals incubated in the presence of the lymphocyte factors had a 50% increase in killing of R. equi, while sensitized macrophages incubated with lymphocyte factors had a > 100% increase in killing capacity.
AB - The interaction of Rhodococcus equi with alveolar macrophages from adult horses, foals experimentally exposed to R. equi (sensitized foals) and non-exposed foals was studied using in vitro bactericidal assays, cytochemical staining and transmission electron microscopy. It was demonstrated that R. equi is a facultative intracellular parasite, able to survive and multiply within the alveolar macrophages of the host by interfering with phagosome-lysosome fusion. Opsonization of R. equi with antibody against capsular components was associated with increased phagosome-lysosome fusion and significantly enhanced (P<0.05) killing of the organism by alveolar macrophages from non-exposed foals. Macrophages from non-exposed foals were able to ingest the non-opsonized organism, but unable to kill > 65% of the infective dose by 6 h post-exposure. Alveolar macrophages from sensitized foals behaved as adult macrophages, able to kill > 95% of the infective dose by 6 h. Lymphocyte factors, derived by in vitro incubation of sensitized peripheral blood lymphocytes with R. equi surface antigens, enhanced macrophage bactericidal activity. Macrophages from non-exposed foals incubated in the presence of the lymphocyte factors had a 50% increase in killing of R. equi, while sensitized macrophages incubated with lymphocyte factors had a > 100% increase in killing capacity.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023389003&partnerID=8YFLogxK
U2 - 10.1016/0378-1135(87)90118-0
DO - 10.1016/0378-1135(87)90118-0
M3 - Article
C2 - 3672873
AN - SCOPUS:0023389003
VL - 14
SP - 307
EP - 320
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 3
ER -