Interaction of nitrogen dioxide with human plasma Antioxidant depletion and oxidative damage

Barry Halliwell, Miao Lin Hu, Samuel Louie, Timothy R. Duvall, Brian K. Tarkington, Paul Motchnik, Carroll E Cross

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

Nitrogen dioxide (NO*2) is often present in inhaled air and may be generated in vivo from nitric oxide. Exposure of human blood plasma to NO*2 caused rapid losses of ascorbic acid, uric acid and protein thiol groups, as well as lipid peroxidation and depletions of α-tocopherol, bilirubin and ubiquinol-10. No increase in protein carbonyls was detected. Supplementation of plasma with ascorbate decreased the rates of lipid peroxidation. α-tocopherol depletion and loss of uric acid. Uric acid supplementation decreased rates of lipid peroxidation but not the loss of α-tecopherol. We conclude that ascorbic acid, protein -SH groups, uric acid and α-tocopherol may be important agents protecting against NO*2 in vivo. If these antioxidants are depleted, peroxidation of lipids occurs and might contribute to the toxicity of NO*2.

Original languageEnglish (US)
Pages (from-to)62-66
Number of pages5
JournalFEBS Letters
Volume313
Issue number1
DOIs
StatePublished - Nov 16 1992

Keywords

  • Antioxidant
  • Lipid peroxidation, Ascorbic acid
  • Nitric oxide
  • Nitrogen dioxide
  • Uric acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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