Interaction of myoglobin with oleic acid

Lifan Shih, Youngran Chung, Renuka Sriram, Thomas Jue

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Previous studies have shown that palmitate (PA) can interact with myoglobin (Mb). The present study has investigated the interaction of the more soluble unsaturated fatty acid, oleic acid (OA). Indeed, <sup>1</sup>H NMR measurements of the Mb signal during OA titration also show signal changes consistent with specific and non-specific binding. At OA:Mb ratio < 4:1, OA perturbs selectively the 8-heme methyl signal, consistent with a local and specific fatty acid-protein interaction. As OA:Mb ratio increases from 4:1 to 40:1, all hyperfine shifted MbCN signals decrease, consistent with a non-selective, global perturbation of the protein. The pH titration analysis indicates that the observed OA methylene signal in the presence of Mb reflects a non-specific interaction and does not originate from a shift in the lamella-micelle equilibrium. Given the OA interaction with Mb, a fatty acid flux model suggests that Mb can play a fatty acid transport role under certain physiological conditions.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalChemistry and Physics of Lipids
Volume191
DOIs
StatePublished - Oct 1 2015

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Keywords

  • Bioenergetics
  • Fatty acid
  • Lipid
  • Lipidprotein interaction
  • Metabolism
  • NMR

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

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