Interaction of MDM33 with mitochondrial inner membrane homeostasis pathways in yeast

Till Klecker, Megan Wemmer, Mathias Haag, Alfons Weig, Stefan Bockler, Thomas Langer, Jodi Nunnari, Benedikt Westermann

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Membrane homeostasis affects mitochondrial dynamics, morphology, and function. Here we report genetic and proteomic data that reveal multiple interactions of Mdm33, a protein essential for normal mitochondrial structure, with components of phospholipid metabolism and mitochondrial inner membrane homeostasis. We screened for suppressors of MDM33 overexpression-induced growth arrest and isolated binding partners by immunoprecipitation of cross-linked cell extracts. These approaches revealed genetic and proteomic interactions of Mdm33 with prohibitins, Phb1 and Phb2, which are key components of mitochondrial inner membrane homeostasis. Lipid profiling by mass spectrometry of mitochondria isolated from Mdm33-overexpressing cells revealed that high levels of Mdm33 affect the levels of phosphatidylethanolamine and cardiolipin, the two key inner membrane phospholipids. Furthermore, we show that cells lacking Mdm33 show strongly decreased mitochondrial fission activity indicating that Mdm33 is critical for mitochondrial membrane dynamics. Our data suggest that MDM33 functionally interacts with components important for inner membrane homeostasis and thereby supports mitochondrial division.

Original languageEnglish (US)
Article number18344
JournalScientific Reports
StatePublished - Dec 16 2015

ASJC Scopus subject areas

  • General


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