Interaction of fatty acid with myoglobin

Renuka Sriram, Ulrike Kreutzer, Lifan Shih, Thomas Jue

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Upon titration with palmitate, the 1H NMR spectra of metmyoglobin cyanide (MbCN) reveal a selective perturbation of the 8 heme methyl, consistent with a specific interaction of myoglobin (Mb) with fatty acid. Other detectable hyperfine shifted resonances of the heme group remain unchanged. Mb also enhances fatty acid solubility, as reflected in a more intense methylene peak of palmitate in Mb solution than in Tris buffer. Ligand binding analysis indicates an apparent palmitate dissociation constant (Kd) of 43 μM. These results suggest that Mb can bind fatty acid and may have a role in facilitating fatty acid transport in the cell.

Original languageEnglish (US)
Pages (from-to)3643-3649
Number of pages7
JournalFEBS Letters
Volume582
Issue number25-26
DOIs
StatePublished - Oct 29 2008

Keywords

  • Bioenergetics
  • Lipid
  • Metabolism
  • NMR

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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