Interaction of fatty acid with myoglobin

Renuka Sriram, Ulrike Kreutzer, Lifan Shih, Thomas Jue

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Upon titration with palmitate, the 1H NMR spectra of metmyoglobin cyanide (MbCN) reveal a selective perturbation of the 8 heme methyl, consistent with a specific interaction of myoglobin (Mb) with fatty acid. Other detectable hyperfine shifted resonances of the heme group remain unchanged. Mb also enhances fatty acid solubility, as reflected in a more intense methylene peak of palmitate in Mb solution than in Tris buffer. Ligand binding analysis indicates an apparent palmitate dissociation constant (Kd) of 43 μM. These results suggest that Mb can bind fatty acid and may have a role in facilitating fatty acid transport in the cell.

Original languageEnglish (US)
Pages (from-to)3643-3649
Number of pages7
JournalFEBS Letters
Issue number25-26
StatePublished - Oct 29 2008


  • Bioenergetics
  • Lipid
  • Metabolism
  • NMR

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology


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