Abstract
The 5′ end of avian sarcoma and leukosis virus RNA near the primer binding site forms two RNA secondary structures, U5-inverted repeat (U5-IR) and U5-leader stems, which are required for efficient initiation of reverse transcription. Lying between these two secondary structures is a 7-base sequence that can anneal to the TφC loop of the tRNATrp primer. Base substitutions in U5 RNA which disrupt this potential interaction result in a defect in the initiation of reverse transcription both in vivo and in vitro. The defect can be complemented in vitro by base substitutions in the primer. The U5 RNA-TφC interaction is also dependent upon the presence of both the U5-IR and the U5-leader structures. These RNA secondary structures and primer interactions are conserved in other type C and D retroviruses, suggesting that there is a common mechanism for the initiation of reverse transcription in all of these retroviruses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2464-2472 |
| Number of pages | 9 |
| Journal | Journal of Virology |
| Volume | 66 |
| Issue number | 4 |
| State | Published - Apr 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Interaction between retroviral U5 RNA and the TφC loop of the tRNATrp primer is required for efficient initiation of reverse transcription. / Aiyar, Ashok; Cobrinik, David; Ge, Zheng; Kung, Hsing-Jien; Leis, Jonathan.
In: Journal of Virology, Vol. 66, No. 4, 04.1992, p. 2464-2472.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interaction between retroviral U5 RNA and the TφC loop of the tRNATrp primer is required for efficient initiation of reverse transcription
AU - Aiyar, Ashok
AU - Cobrinik, David
AU - Ge, Zheng
AU - Kung, Hsing-Jien
AU - Leis, Jonathan
PY - 1992/4
Y1 - 1992/4
N2 - The 5′ end of avian sarcoma and leukosis virus RNA near the primer binding site forms two RNA secondary structures, U5-inverted repeat (U5-IR) and U5-leader stems, which are required for efficient initiation of reverse transcription. Lying between these two secondary structures is a 7-base sequence that can anneal to the TφC loop of the tRNATrp primer. Base substitutions in U5 RNA which disrupt this potential interaction result in a defect in the initiation of reverse transcription both in vivo and in vitro. The defect can be complemented in vitro by base substitutions in the primer. The U5 RNA-TφC interaction is also dependent upon the presence of both the U5-IR and the U5-leader structures. These RNA secondary structures and primer interactions are conserved in other type C and D retroviruses, suggesting that there is a common mechanism for the initiation of reverse transcription in all of these retroviruses.
AB - The 5′ end of avian sarcoma and leukosis virus RNA near the primer binding site forms two RNA secondary structures, U5-inverted repeat (U5-IR) and U5-leader stems, which are required for efficient initiation of reverse transcription. Lying between these two secondary structures is a 7-base sequence that can anneal to the TφC loop of the tRNATrp primer. Base substitutions in U5 RNA which disrupt this potential interaction result in a defect in the initiation of reverse transcription both in vivo and in vitro. The defect can be complemented in vitro by base substitutions in the primer. The U5 RNA-TφC interaction is also dependent upon the presence of both the U5-IR and the U5-leader structures. These RNA secondary structures and primer interactions are conserved in other type C and D retroviruses, suggesting that there is a common mechanism for the initiation of reverse transcription in all of these retroviruses.
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UR - http://www.scopus.com/inward/citedby.url?scp=0026578805&partnerID=8YFLogxK
M3 - Article
C2 - 1548772
AN - SCOPUS:0026578805
VL - 66
SP - 2464
EP - 2472
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 4
ER -