Interaction between retroviral U5 RNA and the TφC loop of the tRNATrp primer is required for efficient initiation of reverse transcription

Ashok Aiyar, David Cobrinik, Zheng Ge, Hsing-Jien Kung, Jonathan Leis

Research output: Contribution to journalArticle

116 Scopus citations

Abstract

The 5′ end of avian sarcoma and leukosis virus RNA near the primer binding site forms two RNA secondary structures, U5-inverted repeat (U5-IR) and U5-leader stems, which are required for efficient initiation of reverse transcription. Lying between these two secondary structures is a 7-base sequence that can anneal to the TφC loop of the tRNATrp primer. Base substitutions in U5 RNA which disrupt this potential interaction result in a defect in the initiation of reverse transcription both in vivo and in vitro. The defect can be complemented in vitro by base substitutions in the primer. The U5 RNA-TφC interaction is also dependent upon the presence of both the U5-IR and the U5-leader structures. These RNA secondary structures and primer interactions are conserved in other type C and D retroviruses, suggesting that there is a common mechanism for the initiation of reverse transcription in all of these retroviruses.

Original languageEnglish (US)
Pages (from-to)2464-2472
Number of pages9
JournalJournal of Virology
Volume66
Issue number4
StatePublished - Apr 1992
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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