Abstract
Two month old C57BL/6 mice were placed on three different diets: 1) normal diet (NC; 0.025% cholesterol), 2) hypercholesterolemic Western-type diet (HC-W; 0.2% cholesterol), and 3) hypercholesterolemic Paigen-type diet (HC-P; 1.25% cholesterol plus 0.5% cholic acid). At 6 months of age, the animals underwent ligation of the left carotid artery and were randomly assigned to vehicle (PBS, subcutaneous) or angiotensin II (Ang II; 1.4 mg/kg/day, subcutaneous) treatment for 4 weeks. Low density lipoprotein-cholesterol levels were similarly increased in both HC diets (NC, 4 ± 3 mg/dl; HC-W, 123 ± 17 mg/dl; HC-P, 160 ± 14 mg/dl). However, the levels of high density lipoprotein- cholesterol (HDL-C) were reduced only in animals fed the HC-P diet (NC, 82 ± 6 mg/dl; HC-W, 79 ± 7 mg/dl; HC-P, 58 ± 7 mg/dl). In Ang II-treated mice, carotid artery ligation induced intimal smooth muscle cell proliferation to a similar extent in NC- and HP-W-fed animals. However, a significantly larger intimal area developed in ligated vessels from Ang II-treated mice fed the HC-P diet (3.6-fold higher than in Ang II-treated NC mice). Together, these results show the accelerating effect of mild hypercholesterolemia, reduced HDL-C levels, and Ang II on intimal hyperplasia after carotid artery ligation in mice.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 476-483 |
| Number of pages | 8 |
| Journal | Journal of Lipid Research |
| Volume | 47 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2006 |
Fingerprint
Keywords
- High density lipoprotein
- Smooth muscle cells
- Vascular remodeling
ASJC Scopus subject areas
- Endocrinology
Cite this
Interaction between mild hypercholesterolemia, HDL-cholesterol levels, and angiotensin II in intimal hyperplasia in mice. / Da Cunha, Valdeci; Martin-McNulty, Baby; Vincelette, Jon; Zhang, Lening; Rutledge, John C; Wilson, Dennis W; Vergona, Ronald; Sullivan, Mark E.; Wang, Yi Xin.
In: Journal of Lipid Research, Vol. 47, No. 3, 03.2006, p. 476-483.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interaction between mild hypercholesterolemia, HDL-cholesterol levels, and angiotensin II in intimal hyperplasia in mice
AU - Da Cunha, Valdeci
AU - Martin-McNulty, Baby
AU - Vincelette, Jon
AU - Zhang, Lening
AU - Rutledge, John C
AU - Wilson, Dennis W
AU - Vergona, Ronald
AU - Sullivan, Mark E.
AU - Wang, Yi Xin
PY - 2006/3
Y1 - 2006/3
N2 - Two month old C57BL/6 mice were placed on three different diets: 1) normal diet (NC; 0.025% cholesterol), 2) hypercholesterolemic Western-type diet (HC-W; 0.2% cholesterol), and 3) hypercholesterolemic Paigen-type diet (HC-P; 1.25% cholesterol plus 0.5% cholic acid). At 6 months of age, the animals underwent ligation of the left carotid artery and were randomly assigned to vehicle (PBS, subcutaneous) or angiotensin II (Ang II; 1.4 mg/kg/day, subcutaneous) treatment for 4 weeks. Low density lipoprotein-cholesterol levels were similarly increased in both HC diets (NC, 4 ± 3 mg/dl; HC-W, 123 ± 17 mg/dl; HC-P, 160 ± 14 mg/dl). However, the levels of high density lipoprotein- cholesterol (HDL-C) were reduced only in animals fed the HC-P diet (NC, 82 ± 6 mg/dl; HC-W, 79 ± 7 mg/dl; HC-P, 58 ± 7 mg/dl). In Ang II-treated mice, carotid artery ligation induced intimal smooth muscle cell proliferation to a similar extent in NC- and HP-W-fed animals. However, a significantly larger intimal area developed in ligated vessels from Ang II-treated mice fed the HC-P diet (3.6-fold higher than in Ang II-treated NC mice). Together, these results show the accelerating effect of mild hypercholesterolemia, reduced HDL-C levels, and Ang II on intimal hyperplasia after carotid artery ligation in mice.
AB - Two month old C57BL/6 mice were placed on three different diets: 1) normal diet (NC; 0.025% cholesterol), 2) hypercholesterolemic Western-type diet (HC-W; 0.2% cholesterol), and 3) hypercholesterolemic Paigen-type diet (HC-P; 1.25% cholesterol plus 0.5% cholic acid). At 6 months of age, the animals underwent ligation of the left carotid artery and were randomly assigned to vehicle (PBS, subcutaneous) or angiotensin II (Ang II; 1.4 mg/kg/day, subcutaneous) treatment for 4 weeks. Low density lipoprotein-cholesterol levels were similarly increased in both HC diets (NC, 4 ± 3 mg/dl; HC-W, 123 ± 17 mg/dl; HC-P, 160 ± 14 mg/dl). However, the levels of high density lipoprotein- cholesterol (HDL-C) were reduced only in animals fed the HC-P diet (NC, 82 ± 6 mg/dl; HC-W, 79 ± 7 mg/dl; HC-P, 58 ± 7 mg/dl). In Ang II-treated mice, carotid artery ligation induced intimal smooth muscle cell proliferation to a similar extent in NC- and HP-W-fed animals. However, a significantly larger intimal area developed in ligated vessels from Ang II-treated mice fed the HC-P diet (3.6-fold higher than in Ang II-treated NC mice). Together, these results show the accelerating effect of mild hypercholesterolemia, reduced HDL-C levels, and Ang II on intimal hyperplasia after carotid artery ligation in mice.
KW - High density lipoprotein
KW - Smooth muscle cells
KW - Vascular remodeling
UR - http://www.scopus.com/inward/record.url?scp=33644785829&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644785829&partnerID=8YFLogxK
U2 - 10.1194/jlr.M500341-JLR200
DO - 10.1194/jlr.M500341-JLR200
M3 - Article
C2 - 16371646
AN - SCOPUS:33644785829
VL - 47
SP - 476
EP - 483
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 3
ER -