Interaction between mild hypercholesterolemia, HDL-cholesterol levels, and angiotensin II in intimal hyperplasia in mice

Valdeci Da Cunha, Baby Martin-McNulty, Jon Vincelette, Lening Zhang, John C Rutledge, Dennis W Wilson, Ronald Vergona, Mark E. Sullivan, Yi Xin Wang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Two month old C57BL/6 mice were placed on three different diets: 1) normal diet (NC; 0.025% cholesterol), 2) hypercholesterolemic Western-type diet (HC-W; 0.2% cholesterol), and 3) hypercholesterolemic Paigen-type diet (HC-P; 1.25% cholesterol plus 0.5% cholic acid). At 6 months of age, the animals underwent ligation of the left carotid artery and were randomly assigned to vehicle (PBS, subcutaneous) or angiotensin II (Ang II; 1.4 mg/kg/day, subcutaneous) treatment for 4 weeks. Low density lipoprotein-cholesterol levels were similarly increased in both HC diets (NC, 4 ± 3 mg/dl; HC-W, 123 ± 17 mg/dl; HC-P, 160 ± 14 mg/dl). However, the levels of high density lipoprotein- cholesterol (HDL-C) were reduced only in animals fed the HC-P diet (NC, 82 ± 6 mg/dl; HC-W, 79 ± 7 mg/dl; HC-P, 58 ± 7 mg/dl). In Ang II-treated mice, carotid artery ligation induced intimal smooth muscle cell proliferation to a similar extent in NC- and HP-W-fed animals. However, a significantly larger intimal area developed in ligated vessels from Ang II-treated mice fed the HC-P diet (3.6-fold higher than in Ang II-treated NC mice). Together, these results show the accelerating effect of mild hypercholesterolemia, reduced HDL-C levels, and Ang II on intimal hyperplasia after carotid artery ligation in mice.

Original languageEnglish (US)
Pages (from-to)476-483
Number of pages8
JournalJournal of Lipid Research
Volume47
Issue number3
DOIs
StatePublished - Mar 2006

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Tunica Intima
Nutrition
Hypercholesterolemia
Angiotensin II
HDL Cholesterol
Hyperplasia
Diet
Carotid Arteries
Ligation
Cholesterol
Animals
Cholic Acid
Inbred C57BL Mouse
LDL Cholesterol
Cell proliferation
Smooth Muscle Myocytes
Muscle
Cell Proliferation

Keywords

  • High density lipoprotein
  • Smooth muscle cells
  • Vascular remodeling

ASJC Scopus subject areas

  • Endocrinology

Cite this

Interaction between mild hypercholesterolemia, HDL-cholesterol levels, and angiotensin II in intimal hyperplasia in mice. / Da Cunha, Valdeci; Martin-McNulty, Baby; Vincelette, Jon; Zhang, Lening; Rutledge, John C; Wilson, Dennis W; Vergona, Ronald; Sullivan, Mark E.; Wang, Yi Xin.

In: Journal of Lipid Research, Vol. 47, No. 3, 03.2006, p. 476-483.

Research output: Contribution to journalArticle

Da Cunha, Valdeci ; Martin-McNulty, Baby ; Vincelette, Jon ; Zhang, Lening ; Rutledge, John C ; Wilson, Dennis W ; Vergona, Ronald ; Sullivan, Mark E. ; Wang, Yi Xin. / Interaction between mild hypercholesterolemia, HDL-cholesterol levels, and angiotensin II in intimal hyperplasia in mice. In: Journal of Lipid Research. 2006 ; Vol. 47, No. 3. pp. 476-483.
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AU - Martin-McNulty, Baby

AU - Vincelette, Jon

AU - Zhang, Lening

AU - Rutledge, John C

AU - Wilson, Dennis W

AU - Vergona, Ronald

AU - Sullivan, Mark E.

AU - Wang, Yi Xin

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