Integrins of the starlet sea anemone nematostella vectensis

Qizhi Gong, Katrina Garvey, Chenghao Qian, Isabel Yin, Gary Wong, Richard P Tucker

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Integrins are extracellular matrix receptors composed of α and β subunits. Here we describe two α subunits and four β subunits from the starlet sea anemone Nematostella vectensis. Phylogenetic analysis suggests that the α subunits are most closely related to RGD-and LDV-dependent α subunits of chordates. The β subunits cluster with the previously described β integrins of the hard coral Acropora millepora. The expression of one of the α subunits and three of the β subunits was confirmed by reverse transcription PCR and in situ hybridization. The α subunit is primarily expressed in cells near muscles, by a subset of gastrodermal cells, and in the gonad. The three β subunits each have distinctive patterns of expression: one is concentrated in the gonad and mesenteric filament, another is found in a subset of cells in the epidermis of the oral region and in a subset of gastrodermal cells in the mesenteries, and a third is expressed widely. Changes in expression were also studied 48 h after horizontal transection by quantitative reverse transcription PCR and in situ hybridization. One of the β subunits is expressed 8-fold higher during regeneration, and its expression is observed in cells within both the epidermis and the gastrodermis at the site of regeneration. Our observations confirm that complex patterns of integrin expression were already present in basal metazoans. The integrins expressed in the gonads may play roles in mediating sperm-egg interactions in N. vectensis, while others may play a role in regulating proliferation during regeneration.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalBiological Bulletin
Volume227
Issue number3
StatePublished - Dec 1 2014

Fingerprint

Sea Anemones
Anthozoa
integrins
Integrins
Gonads
Regeneration
gonads
Epidermis
Reverse Transcription
epidermis (animal)
In Situ Hybridization
in situ hybridization
cells
Chordata
Sperm-Ovum Interactions
Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
Mesentery
Acropora millepora
mesentery

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Gong, Q., Garvey, K., Qian, C., Yin, I., Wong, G., & Tucker, R. P. (2014). Integrins of the starlet sea anemone nematostella vectensis. Biological Bulletin, 227(3), 211-220.

Integrins of the starlet sea anemone nematostella vectensis. / Gong, Qizhi; Garvey, Katrina; Qian, Chenghao; Yin, Isabel; Wong, Gary; Tucker, Richard P.

In: Biological Bulletin, Vol. 227, No. 3, 01.12.2014, p. 211-220.

Research output: Contribution to journalArticle

Gong, Q, Garvey, K, Qian, C, Yin, I, Wong, G & Tucker, RP 2014, 'Integrins of the starlet sea anemone nematostella vectensis', Biological Bulletin, vol. 227, no. 3, pp. 211-220.
Gong Q, Garvey K, Qian C, Yin I, Wong G, Tucker RP. Integrins of the starlet sea anemone nematostella vectensis. Biological Bulletin. 2014 Dec 1;227(3):211-220.
Gong, Qizhi ; Garvey, Katrina ; Qian, Chenghao ; Yin, Isabel ; Wong, Gary ; Tucker, Richard P. / Integrins of the starlet sea anemone nematostella vectensis. In: Biological Bulletin. 2014 ; Vol. 227, No. 3. pp. 211-220.
@article{af795f8b58164a67b987fed12275b866,
title = "Integrins of the starlet sea anemone nematostella vectensis",
abstract = "Integrins are extracellular matrix receptors composed of α and β subunits. Here we describe two α subunits and four β subunits from the starlet sea anemone Nematostella vectensis. Phylogenetic analysis suggests that the α subunits are most closely related to RGD-and LDV-dependent α subunits of chordates. The β subunits cluster with the previously described β integrins of the hard coral Acropora millepora. The expression of one of the α subunits and three of the β subunits was confirmed by reverse transcription PCR and in situ hybridization. The α subunit is primarily expressed in cells near muscles, by a subset of gastrodermal cells, and in the gonad. The three β subunits each have distinctive patterns of expression: one is concentrated in the gonad and mesenteric filament, another is found in a subset of cells in the epidermis of the oral region and in a subset of gastrodermal cells in the mesenteries, and a third is expressed widely. Changes in expression were also studied 48 h after horizontal transection by quantitative reverse transcription PCR and in situ hybridization. One of the β subunits is expressed 8-fold higher during regeneration, and its expression is observed in cells within both the epidermis and the gastrodermis at the site of regeneration. Our observations confirm that complex patterns of integrin expression were already present in basal metazoans. The integrins expressed in the gonads may play roles in mediating sperm-egg interactions in N. vectensis, while others may play a role in regulating proliferation during regeneration.",
author = "Qizhi Gong and Katrina Garvey and Chenghao Qian and Isabel Yin and Gary Wong and Tucker, {Richard P}",
year = "2014",
month = "12",
day = "1",
language = "English (US)",
volume = "227",
pages = "211--220",
journal = "Biological Bulletin",
issn = "0006-3185",
publisher = "Marine Biological Laboratory",
number = "3",

}

TY - JOUR

T1 - Integrins of the starlet sea anemone nematostella vectensis

AU - Gong, Qizhi

AU - Garvey, Katrina

AU - Qian, Chenghao

AU - Yin, Isabel

AU - Wong, Gary

AU - Tucker, Richard P

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Integrins are extracellular matrix receptors composed of α and β subunits. Here we describe two α subunits and four β subunits from the starlet sea anemone Nematostella vectensis. Phylogenetic analysis suggests that the α subunits are most closely related to RGD-and LDV-dependent α subunits of chordates. The β subunits cluster with the previously described β integrins of the hard coral Acropora millepora. The expression of one of the α subunits and three of the β subunits was confirmed by reverse transcription PCR and in situ hybridization. The α subunit is primarily expressed in cells near muscles, by a subset of gastrodermal cells, and in the gonad. The three β subunits each have distinctive patterns of expression: one is concentrated in the gonad and mesenteric filament, another is found in a subset of cells in the epidermis of the oral region and in a subset of gastrodermal cells in the mesenteries, and a third is expressed widely. Changes in expression were also studied 48 h after horizontal transection by quantitative reverse transcription PCR and in situ hybridization. One of the β subunits is expressed 8-fold higher during regeneration, and its expression is observed in cells within both the epidermis and the gastrodermis at the site of regeneration. Our observations confirm that complex patterns of integrin expression were already present in basal metazoans. The integrins expressed in the gonads may play roles in mediating sperm-egg interactions in N. vectensis, while others may play a role in regulating proliferation during regeneration.

AB - Integrins are extracellular matrix receptors composed of α and β subunits. Here we describe two α subunits and four β subunits from the starlet sea anemone Nematostella vectensis. Phylogenetic analysis suggests that the α subunits are most closely related to RGD-and LDV-dependent α subunits of chordates. The β subunits cluster with the previously described β integrins of the hard coral Acropora millepora. The expression of one of the α subunits and three of the β subunits was confirmed by reverse transcription PCR and in situ hybridization. The α subunit is primarily expressed in cells near muscles, by a subset of gastrodermal cells, and in the gonad. The three β subunits each have distinctive patterns of expression: one is concentrated in the gonad and mesenteric filament, another is found in a subset of cells in the epidermis of the oral region and in a subset of gastrodermal cells in the mesenteries, and a third is expressed widely. Changes in expression were also studied 48 h after horizontal transection by quantitative reverse transcription PCR and in situ hybridization. One of the β subunits is expressed 8-fold higher during regeneration, and its expression is observed in cells within both the epidermis and the gastrodermis at the site of regeneration. Our observations confirm that complex patterns of integrin expression were already present in basal metazoans. The integrins expressed in the gonads may play roles in mediating sperm-egg interactions in N. vectensis, while others may play a role in regulating proliferation during regeneration.

UR - http://www.scopus.com/inward/record.url?scp=84920923213&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920923213&partnerID=8YFLogxK

M3 - Article

VL - 227

SP - 211

EP - 220

JO - Biological Bulletin

JF - Biological Bulletin

SN - 0006-3185

IS - 3

ER -