Abstract
Epithelial cells polarize in response to cell-substratum and cell-cell adhesive interactions. Contacts between cells and proteins of the extracellular matrix are mediated by integrin receptors. Of the 24 recognized integrin heterodimers, epithelial cells typically express four or more distinct integrins, with the exact complement dependent on the tissue of origin. Investigation of the roles of integrins in epithelial cell polarization has depended on the use of function-blocking antibodies both to determine ligand specificity of individual integrins and to disrupt and redirect normal morphogenesis. In this article we describe techniques for employing function-blocking anti-integrin antibodies in adhesion assays of the polarized Madin-Darby canine kidney (MDCK) cell line and to demonstrate the involvement of β1 integrins in collagen-induced tubulocyst formation. These techniques can be easily expanded to other antibodies and epithelial cell lines to characterize specific functions of individual integrins in epithelial morphogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 235-246 |
| Number of pages | 12 |
| Journal | Methods |
| Volume | 30 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 1 2003 |
| Externally published | Yes |
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Keywords
- Adhesion assays
- Collagen gels
- Epithelial polarity
- Extracellular matrix
- Function-blocking antibodies
- Integrins
ASJC Scopus subject areas
- Molecular Biology
Cite this
Integrins in epithelial cell polarity : Using antibodies to analyze adhesive function and morphogenesis. / Matlin, Karl S.; Haus, Brian; Zuk, Anna.
In: Methods, Vol. 30, No. 3, 01.07.2003, p. 235-246.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Integrins in epithelial cell polarity
T2 - Using antibodies to analyze adhesive function and morphogenesis
AU - Matlin, Karl S.
AU - Haus, Brian
AU - Zuk, Anna
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Epithelial cells polarize in response to cell-substratum and cell-cell adhesive interactions. Contacts between cells and proteins of the extracellular matrix are mediated by integrin receptors. Of the 24 recognized integrin heterodimers, epithelial cells typically express four or more distinct integrins, with the exact complement dependent on the tissue of origin. Investigation of the roles of integrins in epithelial cell polarization has depended on the use of function-blocking antibodies both to determine ligand specificity of individual integrins and to disrupt and redirect normal morphogenesis. In this article we describe techniques for employing function-blocking anti-integrin antibodies in adhesion assays of the polarized Madin-Darby canine kidney (MDCK) cell line and to demonstrate the involvement of β1 integrins in collagen-induced tubulocyst formation. These techniques can be easily expanded to other antibodies and epithelial cell lines to characterize specific functions of individual integrins in epithelial morphogenesis.
AB - Epithelial cells polarize in response to cell-substratum and cell-cell adhesive interactions. Contacts between cells and proteins of the extracellular matrix are mediated by integrin receptors. Of the 24 recognized integrin heterodimers, epithelial cells typically express four or more distinct integrins, with the exact complement dependent on the tissue of origin. Investigation of the roles of integrins in epithelial cell polarization has depended on the use of function-blocking antibodies both to determine ligand specificity of individual integrins and to disrupt and redirect normal morphogenesis. In this article we describe techniques for employing function-blocking anti-integrin antibodies in adhesion assays of the polarized Madin-Darby canine kidney (MDCK) cell line and to demonstrate the involvement of β1 integrins in collagen-induced tubulocyst formation. These techniques can be easily expanded to other antibodies and epithelial cell lines to characterize specific functions of individual integrins in epithelial morphogenesis.
KW - Adhesion assays
KW - Collagen gels
KW - Epithelial polarity
KW - Extracellular matrix
KW - Function-blocking antibodies
KW - Integrins
UR - http://www.scopus.com/inward/record.url?scp=0037941157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037941157&partnerID=8YFLogxK
U2 - 10.1016/S1046-2023(03)00030-6
DO - 10.1016/S1046-2023(03)00030-6
M3 - Article
C2 - 12798138
AN - SCOPUS:0037941157
VL - 30
SP - 235
EP - 246
JO - Methods
JF - Methods
SN - 0076-6879
IS - 3
ER -