Integration of bacteriophage λ into the cryptic lambdoid prophages of Escherichia coli

A. Lichens-Park, C. L. Smith, Michael Syvanen

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Bacteriophage lambda missing its chromosomal attachment site will integrate into recA+ Escherichia coli K-12 and C at the sites of cryptic prophages. The specific regions in which these recombination events occur were identified in both lambda and the bacterial chromosomes. A NotI restriction site on the prophage allowed its physical mapping. This allowed us to identify the locations of Rac, Qin, and Qsr' cryptic prophages on the NotI map of E. coli K-12 and, by analogy, to identify the cryptic prophage in E. coli C as Qin. No new cryptic prophages were detected in E. coli K-12.

Original languageEnglish (US)
Pages (from-to)2201-2208
Number of pages8
JournalJournal of Bacteriology
Volume172
Issue number5
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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