Integrating multiple analytical datasets to compare metabolite profiles of mouse colonic-cecal contents and feces

Huawei Zeng, Dmitry Grapov, Matthew I. Jackson, Johannes Fahrmann, Oliver Fiehn, Gerald F. Combs

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The pattern of metabolites produced by the gut microbiome comprises a phenotype indicative of the means by which that microbiome affects the gut. We characterized that phenotype in mice by conducting metabolomic analyses of the colonic-cecal contents, comparing that to the metabolite patterns of feces in order to determine the suitability of fecal specimens as proxies for assessing the metabolic impact of the gut microbiome. We detected a total of 270 low molecular weight metabolites in colonic-cecal contents and feces by gas chromatograph, time-of-flight mass spectrometry (GC-TOF) and ultra-high performance liquid chromatography, quadrapole time-of-flight mass spectrometry (UPLC-Q-TOF). Of that number, 251 (93%) were present in both types of specimen, representing almost all known biochemical pathways related to the amino acid, carbohydrate, energy, lipid, membrane transport, nucleotide, genetic information processing, and cancer-related metabolism. A total of 115 metabolites differed significantly in relative abundance between both colonic-cecal contents and feces. These data comprise the first characterization of relationships among metabolites present in the colonic-cecal contents and feces in a healthy mouse model, and shows that feces can be a useful proxy for assessing the pattern of metabolites to which the colonic mucosum is exposed.

Original languageEnglish (US)
Pages (from-to)489-501
Number of pages13
JournalMetabolites
Volume5
Issue number3
DOIs
StatePublished - Sep 11 2015

Fingerprint

Metabolites
Feces
Proxy
Mass Spectrometry
Mass spectrometry
Phenotype
Metabolomics
Membrane Lipids
Automatic Data Processing
High performance liquid chromatography
Metabolism
Nucleotides
Molecular Weight
Gases
High Pressure Liquid Chromatography
Carbohydrates
Datasets
Amino Acids
Molecular weight
Gastrointestinal Microbiome

Keywords

  • Cecal contents
  • Colon
  • Feces
  • Mass spectrometry
  • Metabolite

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

Cite this

Integrating multiple analytical datasets to compare metabolite profiles of mouse colonic-cecal contents and feces. / Zeng, Huawei; Grapov, Dmitry; Jackson, Matthew I.; Fahrmann, Johannes; Fiehn, Oliver; Combs, Gerald F.

In: Metabolites, Vol. 5, No. 3, 11.09.2015, p. 489-501.

Research output: Contribution to journalArticle

Zeng, Huawei ; Grapov, Dmitry ; Jackson, Matthew I. ; Fahrmann, Johannes ; Fiehn, Oliver ; Combs, Gerald F. / Integrating multiple analytical datasets to compare metabolite profiles of mouse colonic-cecal contents and feces. In: Metabolites. 2015 ; Vol. 5, No. 3. pp. 489-501.
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