Integrated proteomic and transcriptomic analysis of differential expression of chicken lung tissue in response to NDV infection during heat stress

Perot Saelao, Ying Wang, Ganrea Chanthavixay, Vivian Yu, Rodrigo A Gallardo, Jack C.M. Dekkers, Susan J. Lamont, Terra Kelly, Huaijun Zhou

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stress, in order to understand the genetic mechanisms of resistance during high ambient temperatures. Fayoumis, which are relatively more resistant to pathogens than Leghorns, had larger numbers of differentially expressed genes (DEGs) during the early stages of infection when compared to Leghorns and subsequently down-regulated their immune response at the latter stages to return to homeostasis. Leghorns had very few DEGs across all observed time points, with the majority of DEGs involved with metabolic and glucose-related functions. Proteomic analysis corroborates findings made within Leghorns, while also identifying interesting candidate genes missed by expression profiling. Poor correlation between changes observed in the proteomic and transcriptomic datasets highlights the potential importance of integrative approaches to understand the mechanisms of disease response. Overall, this study provides novel insights into global protein and expression profiles of these two genetic lines, and provides potential genetic targets involved with NDV resistance during heat stress in poultry.

Original languageEnglish (US)
Article number579
JournalGenes
Volume9
Issue number12
DOIs
StatePublished - Dec 1 2018

Fingerprint

Newcastle disease virus
Virus Diseases
Proteomics
Chickens
Hot Temperature
Poultry
Lung
Genes
Disease Resistance
Food Supply
Gene Expression Profiling
Homeostasis
Glucose
Temperature
Infection
Proteins

Keywords

  • Chicken
  • Newcastle disease virus
  • Proteomics
  • RNA-seq

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Integrated proteomic and transcriptomic analysis of differential expression of chicken lung tissue in response to NDV infection during heat stress. / Saelao, Perot; Wang, Ying; Chanthavixay, Ganrea; Yu, Vivian; Gallardo, Rodrigo A; Dekkers, Jack C.M.; Lamont, Susan J.; Kelly, Terra; Zhou, Huaijun.

In: Genes, Vol. 9, No. 12, 579, 01.12.2018.

Research output: Contribution to journalArticle

Saelao, Perot ; Wang, Ying ; Chanthavixay, Ganrea ; Yu, Vivian ; Gallardo, Rodrigo A ; Dekkers, Jack C.M. ; Lamont, Susan J. ; Kelly, Terra ; Zhou, Huaijun. / Integrated proteomic and transcriptomic analysis of differential expression of chicken lung tissue in response to NDV infection during heat stress. In: Genes. 2018 ; Vol. 9, No. 12.
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