Integrated epigenomic analyses of neuronal MeCP2 reveal a role for long-range interaction with active genes

Dag H. Yasui, Sailaja Peddada, Mark C. Bieda, Roxanne O. Vallero, Amber Hogart, Raman P. Nagarajan, Karen N. Thatcher, Peggy J. Farnham, Janine M LaSalle

Research output: Contribution to journalArticle

279 Citations (Scopus)

Abstract

Mutations in MECP2 cause the autism-spectrum disorder Rett syndrome. MeCP2 is predicted to bind to methylated promoters and silence transcription. However, the first large-scale mapping of neuronal MeCP2-binding sites on 26.3 Mb of imprinted and non-imprinted loci revealed that 59% of MeCP2-binding sites are outside of genes and that only 6% are in CpG islands. Integrated genome-wide promoter analysis of MeCP2 binding, CpG methylation, and gene expression revealed that 63% of MeCP2-bound promoters are actively expressed and that only 6% are highly methylated. These results indicate that the primary function of MeCP2 is not the silencing of methylated promoters.

Original languageEnglish (US)
Pages (from-to)19416-19421
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number49
DOIs
StatePublished - Dec 4 2007

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Epigenomics
Binding Sites
Rett Syndrome
CpG Islands
Methylation
Genes
Genome
Gene Expression
Mutation
Autism Spectrum Disorder

Keywords

  • Chromatin
  • DNA methylation
  • Epigenetics
  • Genomics
  • Rett syndrome

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Integrated epigenomic analyses of neuronal MeCP2 reveal a role for long-range interaction with active genes. / Yasui, Dag H.; Peddada, Sailaja; Bieda, Mark C.; Vallero, Roxanne O.; Hogart, Amber; Nagarajan, Raman P.; Thatcher, Karen N.; Farnham, Peggy J.; LaSalle, Janine M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 49, 04.12.2007, p. 19416-19421.

Research output: Contribution to journalArticle

Yasui, Dag H. ; Peddada, Sailaja ; Bieda, Mark C. ; Vallero, Roxanne O. ; Hogart, Amber ; Nagarajan, Raman P. ; Thatcher, Karen N. ; Farnham, Peggy J. ; LaSalle, Janine M. / Integrated epigenomic analyses of neuronal MeCP2 reveal a role for long-range interaction with active genes. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 49. pp. 19416-19421.
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AU - Hogart, Amber

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