Integrated bovine leukosis proviral DNA in T helper and T cytotoxic/suppressor lymphocytes

M. L. Stott, Mark Thurmond, S. J. Dunn, Bennie Osburn, Jeffrey L Stott

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Bovine leukosis virus (BLV) is associated with the disease complex enzootic bovine leukosis. The infection may remain clinically silent in the form of an aleukaemic state or emerge as a persistent lymphocytosis and more rarely as lymphosarcoma. BLV has been considered classically to be a B lymphotropic virus, based upon the absolute increase in B lymphocytes in persistent lymphocytosis, the B lymphocyte phenotype of a majority of the cells making up lymphosarcomas and the identification of viral antigen expressed in B lymphocytes following in vitro culture of peripheral blood mononuclear leukocytes. This association of BLV with B lymphocytes is well established but the mechanism(s) of disease expression is not defined. To examine further the cellular tropism(s) of BLV, T lymphocyte subpopulations from 10 lymphocytotic cattle were established in vitro. Lymphocyte cultures were characterized by their subpopulation phenotype and DNA was extracted for identification of integrated provirus by Southern blot hybridization. Provirus was identified in T lymphocyte cultures derived from seven of 10 lymphocytotic cattle, with both T helper and T cytotoxic/suppressor subpopulations affected.

Original languageEnglish (US)
Pages (from-to)307-315
Number of pages9
JournalJournal of General Virology
Volume72
Issue number2
StatePublished - 1991

ASJC Scopus subject areas

  • Virology
  • Immunology

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