Integrated approach to designing growth factor delivery systems

Ruth R. Chen, Eduardo Silva, William W. Yuen, Andrea A. Brock, Claudia Fischbach, Angela S. Lin, Robert E. Guldberg, David J. Mooney

Research output: Contribution to journalArticle

110 Scopus citations

Abstract

Growth factors have been widely used in strategies to regenerate and repair diseased tissues, but current therapies that go directly from bench to bedside have had limited clinical success. We hypothesize that engineering successful therapies with recombinant proteins will often require specific quantitative information of the spatiotemporal role of the factors and the development of sophisticated delivery approaches that provide appropriate tissue exposures. This hypothesis was tested in the context of therapeutic angiogenesis. An in vitro model of angiogenesis was adapted to quantify the role of the concentration/gradient of vascular endothelial growth factor [VEGF(165)] on microvascular endothelial cells, and a delivery system was then designed, based on a mathematical model, to provide the desired profile in ischemic mice hindlimbs. This system significantly enhanced blood vessel formation, and perfusion and recovery from severe ischemia. This general approach may be broadly applicable to growth factor therapies.

Original languageEnglish (US)
Pages (from-to)3896-3903
Number of pages8
JournalFASEB Journal
Volume21
Issue number14
DOIs
StatePublished - Dec 1 2007
Externally publishedYes

Keywords

  • Angiogenesis
  • In vitro models
  • Tissue engineering

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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    Chen, R. R., Silva, E., Yuen, W. W., Brock, A. A., Fischbach, C., Lin, A. S., Guldberg, R. E., & Mooney, D. J. (2007). Integrated approach to designing growth factor delivery systems. FASEB Journal, 21(14), 3896-3903. https://doi.org/10.1096/fj.06-7873com