Integrated approach to designing growth factor delivery systems

Ruth R. Chen, Eduardo Silva, William W. Yuen, Andrea A. Brock, Claudia Fischbach, Angela S. Lin, Robert E. Guldberg, David J. Mooney

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Growth factors have been widely used in strategies to regenerate and repair diseased tissues, but current therapies that go directly from bench to bedside have had limited clinical success. We hypothesize that engineering successful therapies with recombinant proteins will often require specific quantitative information of the spatiotemporal role of the factors and the development of sophisticated delivery approaches that provide appropriate tissue exposures. This hypothesis was tested in the context of therapeutic angiogenesis. An in vitro model of angiogenesis was adapted to quantify the role of the concentration/gradient of vascular endothelial growth factor [VEGF(165)] on microvascular endothelial cells, and a delivery system was then designed, based on a mathematical model, to provide the desired profile in ischemic mice hindlimbs. This system significantly enhanced blood vessel formation, and perfusion and recovery from severe ischemia. This general approach may be broadly applicable to growth factor therapies.

Original languageEnglish (US)
Pages (from-to)3896-3903
Number of pages8
JournalFASEB Journal
Volume21
Issue number14
DOIs
StatePublished - Dec 1 2007
Externally publishedYes

Fingerprint

growth factors
Intercellular Signaling Peptides and Proteins
Tissue
therapeutics
Endothelial cells
Blood vessels
angiogenesis
Recombinant Proteins
Vascular Endothelial Growth Factor A
Repair
Hindlimb
Mathematical models
Cell- and Tissue-Based Therapy
Recovery
Blood Vessels
vascular endothelial growth factors
Theoretical Models
Therapeutics
Ischemia
Endothelial Cells

Keywords

  • Angiogenesis
  • In vitro models
  • Tissue engineering

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Chen, R. R., Silva, E., Yuen, W. W., Brock, A. A., Fischbach, C., Lin, A. S., ... Mooney, D. J. (2007). Integrated approach to designing growth factor delivery systems. FASEB Journal, 21(14), 3896-3903. https://doi.org/10.1096/fj.06-7873com

Integrated approach to designing growth factor delivery systems. / Chen, Ruth R.; Silva, Eduardo; Yuen, William W.; Brock, Andrea A.; Fischbach, Claudia; Lin, Angela S.; Guldberg, Robert E.; Mooney, David J.

In: FASEB Journal, Vol. 21, No. 14, 01.12.2007, p. 3896-3903.

Research output: Contribution to journalArticle

Chen, RR, Silva, E, Yuen, WW, Brock, AA, Fischbach, C, Lin, AS, Guldberg, RE & Mooney, DJ 2007, 'Integrated approach to designing growth factor delivery systems', FASEB Journal, vol. 21, no. 14, pp. 3896-3903. https://doi.org/10.1096/fj.06-7873com
Chen RR, Silva E, Yuen WW, Brock AA, Fischbach C, Lin AS et al. Integrated approach to designing growth factor delivery systems. FASEB Journal. 2007 Dec 1;21(14):3896-3903. https://doi.org/10.1096/fj.06-7873com
Chen, Ruth R. ; Silva, Eduardo ; Yuen, William W. ; Brock, Andrea A. ; Fischbach, Claudia ; Lin, Angela S. ; Guldberg, Robert E. ; Mooney, David J. / Integrated approach to designing growth factor delivery systems. In: FASEB Journal. 2007 ; Vol. 21, No. 14. pp. 3896-3903.
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