Integrated analysis of mRNA and microRNA expression in mature neurons, neural progenitor cells and neuroblastoma cells

Da Liu, Bradley Ander, Yingfang Tian, Boryana Stamova, Glen C. Jickling, Ryan R. Davis, Frank R Sharp

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Mature neurons (MNs), neural progenitor cells (NPCs) and neuroblastoma cells (NBCs) are all neural-derived cells. However, MNs are unable to divide once differentiated; NPCs are able to divide a limited number of times and differentiate to normal brain cell types; whereas NBCs can divide an unlimited number of times but rarely differentiate. Here, we perform whole transcriptome (mRNA, miRNA) profiling of these cell types and compare expression levels of each cell type to the others. Integrated mRNA-miRNA functional analyses reveal that: 1) several very highly expressed genes (e.g., Robo1, Nrp1, Epha3, Unc5c, Dcc, Pak3, Limk4) and a few under-expressed miRNAs (e.g., miR-152, miR-146b, miR-339-5p) in MNs are associated with one important cellular process-axon guidance; 2) some very highly expressed mitogenic pathway genes (e.g., Map2k1, Igf1r, Rara, Runx1) and under-expressed miRNAs (e.g., miR-370, miR-9, miR-672) in NBCs are associated with cancer pathways. These results provide a library of negative mRNAmiRNA networks that are likely involved in the cellular processes of differentiation and division.

Original languageEnglish (US)
Pages (from-to)120-127
Number of pages8
JournalGene
Volume495
Issue number2
DOIs
StatePublished - Mar 10 2012

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MicroRNAs
Neuroblastoma
Stem Cells
Neurons
Messenger RNA
Transcriptome
Genes
Libraries
Brain
Neoplasms

Keywords

  • Mature neurons (MNs)
  • MicroRNA (miRNA)
  • MRNA
  • Neural progenitor cells (NPCs)
  • Neuroblastoma cells (NBCs)

ASJC Scopus subject areas

  • Genetics

Cite this

Integrated analysis of mRNA and microRNA expression in mature neurons, neural progenitor cells and neuroblastoma cells. / Liu, Da; Ander, Bradley; Tian, Yingfang; Stamova, Boryana; Jickling, Glen C.; Davis, Ryan R.; Sharp, Frank R.

In: Gene, Vol. 495, No. 2, 10.03.2012, p. 120-127.

Research output: Contribution to journalArticle

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