Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes

Maurice Wong, Gege Xu, Dayoung Park, Mariana Barboza Gardner, Carlito B Lebrilla

Research output: Contribution to journalArticle

Abstract

Glycosphingolipids (GSLs) are found in cellular membranes of most organisms and play important roles in cell-cell recognition, signaling, growth, and adhesion, among others. A method based on nanoflow high performance liquid chromatography-chip-quadrupole-time-of-flight mass spectrometry (nanoHPLC Chip-Q-TOF MS) was applied towards identifying and quantifying intact GSLs from a variety of samples, including cultured cell lines and animal tissue. The method provides the composition and sequence of the glycan, as well as variations in the ceramide portion of the GSL. It was used to profile the changes in the glycolipidome of Caco-2 cells as they undergo differentiation. A total of 226 unique GSLs were found among Caco-2 samples from five differentiation time-points. The method provided a comprehensive glycolipidomic profile of a cell during differentiation to yield the dynamic variation of intact GSL structures.

Original languageEnglish (US)
Article number10993
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Glycosphingolipids
Cell membranes
Polysaccharides
Lipids
Ceramides
High performance liquid chromatography
Mass spectrometry
Animals
Adhesion
Cells
Tissue
Membranes
Chemical analysis

ASJC Scopus subject areas

  • General

Cite this

Intact glycosphingolipidomic analysis of the cell membrane during differentiation yields extensive glycan and lipid changes. / Wong, Maurice; Xu, Gege; Park, Dayoung; Barboza Gardner, Mariana; Lebrilla, Carlito B.

In: Scientific Reports, Vol. 8, No. 1, 10993, 01.12.2018.

Research output: Contribution to journalArticle

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