Abstract
The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.
Original language | English (US) |
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Pages (from-to) | 155-160 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 388 |
Issue number | 2-3 |
DOIs | |
State | Published - Jun 17 1996 |
Externally published | Yes |
Keywords
- Competitive polymerase chain reaction
- Gastrointestinal tract
- In situ hybridization
- Insulin-like growth factor
- Messenger RNA
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology