Abstract
The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 155-160 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 388 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Jun 17 1996 |
| Externally published | Yes |
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Keywords
- Competitive polymerase chain reaction
- Gastrointestinal tract
- In situ hybridization
- Insulin-like growth factor
- Messenger RNA
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats. / Dvořák, Bohuslav; Stephana, Audrey L.; Holubec, Hana; Williams, Catherine S.; Philipps, Anthony F; Koldovskoý, Otakar.
In: FEBS Letters, Vol. 388, No. 2-3, 17.06.1996, p. 155-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats
AU - Dvořák, Bohuslav
AU - Stephana, Audrey L.
AU - Holubec, Hana
AU - Williams, Catherine S.
AU - Philipps, Anthony F
AU - Koldovskoý, Otakar
PY - 1996/6/17
Y1 - 1996/6/17
N2 - The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.
AB - The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.
KW - Competitive polymerase chain reaction
KW - Gastrointestinal tract
KW - In situ hybridization
KW - Insulin-like growth factor
KW - Messenger RNA
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UR - http://www.scopus.com/inward/citedby.url?scp=0030591435&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(96)00495-4
DO - 10.1016/0014-5793(96)00495-4
M3 - Article
C2 - 8690076
AN - SCOPUS:0030591435
VL - 388
SP - 155
EP - 160
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -