Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats

Bohuslav Dvořák; Audrey L. Stephana; Hana Holubec; Catherine S. Williams; Anthony F Philipps; Otakar Koldovskoý

doi:10.1016/0014-5793(96)00495-4

Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats

Bohuslav Dvořák, Audrey L. Stephana, Hana Holubec, Catherine S. Williams, Anthony F Philipps, Otakar Koldovskoý

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.

Original language	English (US)
Pages (from-to)	155-160
Number of pages	6
Journal	FEBS Letters
Volume	388
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(96)00495-4
State	Published - Jun 17 1996
Externally published	Yes

Keywords

Competitive polymerase chain reaction
Gastrointestinal tract
In situ hybridization
Insulin-like growth factor
Messenger RNA

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(96)00495-4

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title = "Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats",

abstract = "The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.",

keywords = "Competitive polymerase chain reaction, Gastrointestinal tract, In situ hybridization, Insulin-like growth factor, Messenger RNA",

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T1 - Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats

AU - Dvořák, Bohuslav

AU - Stephana, Audrey L.

AU - Holubec, Hana

AU - Williams, Catherine S.

AU - Philipps, Anthony F

AU - Koldovskoý, Otakar

PY - 1996/6/17

Y1 - 1996/6/17

N2 - The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.

AB - The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.

KW - Competitive polymerase chain reaction

KW - Gastrointestinal tract

KW - In situ hybridization

KW - Insulin-like growth factor

KW - Messenger RNA

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Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats

Abstract

Keywords

ASJC Scopus subject areas

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