Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats

Bohuslav Dvořák, Audrey L. Stephana, Hana Holubec, Catherine S. Williams, Anthony F Philipps, Otakar Koldovskoý

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The presence and cellular localization of insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats was studied. A sensitive reverse transcription (RT) competitive-polymerase chain reaction (PCR) revealed IGF-I gene expression in both age groups. Adult tissue contained 3-fold higher levels of IGF-I mRNA in comparison with sucklings. Using an in situ hybridization technique, IGF-I transcripts were localized mainly in enterocytes and goblet cells in the intestinal crypts of adult rats. By using this technique, IGF-I mRNA was not detected in jejunum of 12-day-old rats.

Original languageEnglish (US)
Pages (from-to)155-160
Number of pages6
JournalFEBS Letters
Volume388
Issue number2-3
DOIs
StatePublished - Jun 17 1996
Externally publishedYes

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Keywords

  • Competitive polymerase chain reaction
  • Gastrointestinal tract
  • In situ hybridization
  • Insulin-like growth factor
  • Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Dvořák, B., Stephana, A. L., Holubec, H., Williams, C. S., Philipps, A. F., & Koldovskoý, O. (1996). Insulin-like growth factor-I (IGF-I) mRNA in the small intestine of suckling and adult rats. FEBS Letters, 388(2-3), 155-160. https://doi.org/10.1016/0014-5793(96)00495-4