Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes

Simon Hennig, Sebastian Van De Linde, Martina Lummer, Matthias Simonis, Thomas R Huser, Markus Sauer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm, we deliver functionalized fluorescent probes directly into the cells by (di)electrophoretic forces. The label density can be adjusted and traced directly during the staining process by fluorescence microscopy. We demonstrate the potential of this technique by delivering and imaging a range of commercially available cell-permeable and nonpermeable fluorescent probes to cells.

Original languageEnglish (US)
Pages (from-to)1374-1381
Number of pages8
JournalNano Letters
Volume15
Issue number2
DOIs
StatePublished - Feb 11 2015
Externally publishedYes

Fingerprint

Fluorescent Dyes
staining
Imaging techniques
Labeling
marking
probes
cells
Cells
Fluorescence microscopy
Labels
hollow
microscopy
Glass
fluorescence
glass

Keywords

  • live-cell imaging
  • Nanoinjection
  • nanopipette
  • online monitoring of live-cell labeling
  • scanning ion conductance microscopy
  • super-resolution imaging

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Hennig, S., Van De Linde, S., Lummer, M., Simonis, M., Huser, T. R., & Sauer, M. (2015). Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes. Nano Letters, 15(2), 1374-1381. https://doi.org/10.1021/nl504660t

Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes. / Hennig, Simon; Van De Linde, Sebastian; Lummer, Martina; Simonis, Matthias; Huser, Thomas R; Sauer, Markus.

In: Nano Letters, Vol. 15, No. 2, 11.02.2015, p. 1374-1381.

Research output: Contribution to journalArticle

Hennig, S, Van De Linde, S, Lummer, M, Simonis, M, Huser, TR & Sauer, M 2015, 'Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes', Nano Letters, vol. 15, no. 2, pp. 1374-1381. https://doi.org/10.1021/nl504660t
Hennig, Simon ; Van De Linde, Sebastian ; Lummer, Martina ; Simonis, Matthias ; Huser, Thomas R ; Sauer, Markus. / Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes. In: Nano Letters. 2015 ; Vol. 15, No. 2. pp. 1374-1381.
@article{b63ab45ad4a446e7906bcf9b90fc9cee,
title = "Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes",
abstract = "Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm, we deliver functionalized fluorescent probes directly into the cells by (di)electrophoretic forces. The label density can be adjusted and traced directly during the staining process by fluorescence microscopy. We demonstrate the potential of this technique by delivering and imaging a range of commercially available cell-permeable and nonpermeable fluorescent probes to cells.",
keywords = "live-cell imaging, Nanoinjection, nanopipette, online monitoring of live-cell labeling, scanning ion conductance microscopy, super-resolution imaging",
author = "Simon Hennig and {Van De Linde}, Sebastian and Martina Lummer and Matthias Simonis and Huser, {Thomas R} and Markus Sauer",
year = "2015",
month = "2",
day = "11",
doi = "10.1021/nl504660t",
language = "English (US)",
volume = "15",
pages = "1374--1381",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "2",

}

TY - JOUR

T1 - Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes

AU - Hennig, Simon

AU - Van De Linde, Sebastian

AU - Lummer, Martina

AU - Simonis, Matthias

AU - Huser, Thomas R

AU - Sauer, Markus

PY - 2015/2/11

Y1 - 2015/2/11

N2 - Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm, we deliver functionalized fluorescent probes directly into the cells by (di)electrophoretic forces. The label density can be adjusted and traced directly during the staining process by fluorescence microscopy. We demonstrate the potential of this technique by delivering and imaging a range of commercially available cell-permeable and nonpermeable fluorescent probes to cells.

AB - Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm, we deliver functionalized fluorescent probes directly into the cells by (di)electrophoretic forces. The label density can be adjusted and traced directly during the staining process by fluorescence microscopy. We demonstrate the potential of this technique by delivering and imaging a range of commercially available cell-permeable and nonpermeable fluorescent probes to cells.

KW - live-cell imaging

KW - Nanoinjection

KW - nanopipette

KW - online monitoring of live-cell labeling

KW - scanning ion conductance microscopy

KW - super-resolution imaging

UR - http://www.scopus.com/inward/record.url?scp=84922808500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922808500&partnerID=8YFLogxK

U2 - 10.1021/nl504660t

DO - 10.1021/nl504660t

M3 - Article

C2 - 25533766

AN - SCOPUS:84922808500

VL - 15

SP - 1374

EP - 1381

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 2

ER -