Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes

Simon Hennig, Sebastian Van De Linde, Martina Lummer, Matthias Simonis, Thomas R Huser, Markus Sauer

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm, we deliver functionalized fluorescent probes directly into the cells by (di)electrophoretic forces. The label density can be adjusted and traced directly during the staining process by fluorescence microscopy. We demonstrate the potential of this technique by delivering and imaging a range of commercially available cell-permeable and nonpermeable fluorescent probes to cells.

Original languageEnglish (US)
Pages (from-to)1374-1381
Number of pages8
JournalNano Letters
Volume15
Issue number2
DOIs
StatePublished - Feb 11 2015
Externally publishedYes

Keywords

  • live-cell imaging
  • Nanoinjection
  • nanopipette
  • online monitoring of live-cell labeling
  • scanning ion conductance microscopy
  • super-resolution imaging

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

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  • Cite this

    Hennig, S., Van De Linde, S., Lummer, M., Simonis, M., Huser, T. R., & Sauer, M. (2015). Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes. Nano Letters, 15(2), 1374-1381. https://doi.org/10.1021/nl504660t