Abstract
Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm, we deliver functionalized fluorescent probes directly into the cells by (di)electrophoretic forces. The label density can be adjusted and traced directly during the staining process by fluorescence microscopy. We demonstrate the potential of this technique by delivering and imaging a range of commercially available cell-permeable and nonpermeable fluorescent probes to cells.
Original language | English (US) |
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Pages (from-to) | 1374-1381 |
Number of pages | 8 |
Journal | Nano Letters |
Volume | 15 |
Issue number | 2 |
DOIs | |
State | Published - Feb 11 2015 |
Externally published | Yes |
Keywords
- live-cell imaging
- Nanoinjection
- nanopipette
- online monitoring of live-cell labeling
- scanning ion conductance microscopy
- super-resolution imaging
ASJC Scopus subject areas
- Condensed Matter Physics
- Bioengineering
- Chemistry(all)
- Materials Science(all)
- Mechanical Engineering