Insights into pathogen immune evasion mechanisms: Anaplasma phagocytophilum fails to induce an apoptosis differentiation program in human neutrophils

Dori L Borjesson, Scott D. Kobayashi, Adeline R. Whitney, Jovanka M. Voyich, Cynthia M. Argue, Frank R. DeLeo

Research output: Contribution to journalArticle

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Abstract

Polymorphonuclear leukocytes (PMNs or neutrophils) are essential to human innate host defense. However, some bacterial pathogens circumvent destruction by PMNs and thereby cause disease. Anaplasma phagocytophilum, the agent of human granulocytic anaplasmosis, survives within PMNs in part by altering normal host cell processes, such as production of reactive oxygen species (ROS) and apoptosis. To investigate the molecular basis of A. phagocytophilum survival within neutrophils, we used Affymetrix microarrays to measure global changes in human PMN gene expression following infection with A. phagocytophilum. Notably, A. phagocytophilum uptake induced fewer perturbations in host cell gene regulation compared with phagocytosis of Staphylococcus aureus. Although ingestion of A. phagocytophilum did not elicit significant PMN ROS, proinflammatory genes were gradually up-regulated, indicating delayed PMN activation rather than loss of proinflammatory capacity normally observed during phagocytosis-induced apoptosis. Importantly, ingestion of A. phagocytophilum failed to trigger the neutrophil apoptosis differentiation program that typically follows phagocytosis and ROS production. Heat-killed A. phagocytophilum caused some similar initial alterations in neutrophil gene expression and function, which included delaying normal PMN apoptosis and blocking Fas-induced programmed cell death. However, at 24 h, down-regulation of PMN gene transcription may be more reliant on active infection. Taken together, these findings suggest two separate antiapoptotic processes may work concomitantly to promote bacterial survival: 1) uptake of A. phagocytophilum fails to trigger the apoptosis differentiation program usually induced by bacteria, and 2) a protein or molecule on the pathogen surface can mediate an early delay in spontaneous neutrophil apoptosis.

Original languageEnglish (US)
Pages (from-to)6364-6372
Number of pages9
JournalJournal of Immunology
Volume174
Issue number10
StatePublished - May 15 2005
Externally publishedYes

Fingerprint

Anaplasma phagocytophilum
Immune Evasion
Neutrophils
Apoptosis
Phagocytosis
Reactive Oxygen Species
Eating
Anaplasmosis
Genes
Gene Expression
Survival
Infection
Staphylococcus aureus
Cell Death
Down-Regulation
Hot Temperature
Bacteria

ASJC Scopus subject areas

  • Immunology

Cite this

Insights into pathogen immune evasion mechanisms : Anaplasma phagocytophilum fails to induce an apoptosis differentiation program in human neutrophils. / Borjesson, Dori L; Kobayashi, Scott D.; Whitney, Adeline R.; Voyich, Jovanka M.; Argue, Cynthia M.; DeLeo, Frank R.

In: Journal of Immunology, Vol. 174, No. 10, 15.05.2005, p. 6364-6372.

Research output: Contribution to journalArticle

Borjesson, Dori L ; Kobayashi, Scott D. ; Whitney, Adeline R. ; Voyich, Jovanka M. ; Argue, Cynthia M. ; DeLeo, Frank R. / Insights into pathogen immune evasion mechanisms : Anaplasma phagocytophilum fails to induce an apoptosis differentiation program in human neutrophils. In: Journal of Immunology. 2005 ; Vol. 174, No. 10. pp. 6364-6372.
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