Abstract
An efficient procedure of transforming Arabidopsis thaliana germinating seeds was elaborated on the basis of the method of Feldmann and Marks. The procedure involves microdamaging Tl seeds by sonication before culturing with a vector Agrobacterium strain and yields more than 1% T2 transformants. Germinating seeds were transformed with Agrobacterium timefaciens hypervirulent strain A281 carrying plasmid pLD3 derived from pBI 121. A collection of 54 A. thaliana T2 transformants was obtained; some of them showed marked morphological alterations. The transgenic nature of plants that acquired resistance to a marker antibiotic was confirmed histochemically and by PCR amplification of a T-DNA region.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1214-1222 |
| Number of pages | 9 |
| Journal | Genetika |
| Volume | 35 |
| Issue number | 9 |
| State | Published - Dec 1 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Insertional mutagenesis in arabidopsis thaliana : presonication of germinating seeds increases the efficiency of their transformation. / Tomilov, Alexey; Tomilova, N. B.; Ogarkova, O. A.; Tarasov, V. A.
In: Genetika, Vol. 35, No. 9, 01.12.1999, p. 1214-1222.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Insertional mutagenesis in arabidopsis thaliana
T2 - presonication of germinating seeds increases the efficiency of their transformation
AU - Tomilov, Alexey
AU - Tomilova, N. B.
AU - Ogarkova, O. A.
AU - Tarasov, V. A.
PY - 1999/12/1
Y1 - 1999/12/1
N2 - An efficient procedure of transforming Arabidopsis thaliana germinating seeds was elaborated on the basis of the method of Feldmann and Marks. The procedure involves microdamaging Tl seeds by sonication before culturing with a vector Agrobacterium strain and yields more than 1% T2 transformants. Germinating seeds were transformed with Agrobacterium timefaciens hypervirulent strain A281 carrying plasmid pLD3 derived from pBI 121. A collection of 54 A. thaliana T2 transformants was obtained; some of them showed marked morphological alterations. The transgenic nature of plants that acquired resistance to a marker antibiotic was confirmed histochemically and by PCR amplification of a T-DNA region.
AB - An efficient procedure of transforming Arabidopsis thaliana germinating seeds was elaborated on the basis of the method of Feldmann and Marks. The procedure involves microdamaging Tl seeds by sonication before culturing with a vector Agrobacterium strain and yields more than 1% T2 transformants. Germinating seeds were transformed with Agrobacterium timefaciens hypervirulent strain A281 carrying plasmid pLD3 derived from pBI 121. A collection of 54 A. thaliana T2 transformants was obtained; some of them showed marked morphological alterations. The transgenic nature of plants that acquired resistance to a marker antibiotic was confirmed histochemically and by PCR amplification of a T-DNA region.
UR - http://www.scopus.com/inward/record.url?scp=0033183418&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033183418&partnerID=8YFLogxK
M3 - Article
C2 - 10576057
AN - SCOPUS:0033183418
VL - 35
SP - 1214
EP - 1222
JO - Genetika
JF - Genetika
SN - 0016-6758
IS - 9
ER -