Insecticidal properties of genetically engineered baculoviruses expressing an insect juvenile hormone esterase gene

R. Eldridge, D. R. O'Reilly, B. D. Hammock, L. K. Miller

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Exploring the possibility of enhancing the properties of baculoviruses as biological control agents of insect pests, we tested the effect of expressing an insect gene (jhe) encoding juvenile hormone esterase. Juvenile hormone esterase inactivates juvenile hormone, which regulates the outcome of an insect molt. A cDNA encoding the juvenile hormone esterase of Heliothis virescens was inserted into the genome of Autographa californica nuclear polyhedrosis virus such that the gene was expressed under the control of a strong, modified viral promoter. This virus, however, naturally encodes an ecdysteroid UDP-glucosyltransferase which inactivates ecdysone, the hormone which initiates molting. Since ecdysteroid UDP-glucosyltransferase could mask the effects of jhe expression by blocking molting entirely, jhe-expressing viruses in which the ecdysteroid UDP-glucosyltransferase gene was deleted or disrupted were constructed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of proteins from infected cells revealed several intracellular proteins and two major secreted proteins which reacted with antibodies to authentic juvenile hormone esterase. Western blot analysis coupled with tunicamycin treatment indicated that differential glycosylation was responsible for the multiple products. Hemolymph of recombinant virus- infected fourth-instar Trichoplusia ni larvae contained levels of juvenile hormone esterase activity 40-fold higher than maximal levels found in uninfected larvae. However, little or no difference in developmental characteristics, weight gain, or time of mortality was observed between insects infected with the jhe-expressing viruses and control viruses.

Original languageEnglish (US)
Pages (from-to)1583-1591
Number of pages9
JournalApplied and Environmental Microbiology
Volume58
Issue number5
StatePublished - 1992
Externally publishedYes

Fingerprint

Insect Hormones
juvenile hormone esterase
insect hormones
insecticidal properties
Baculoviridae
hormone
glucosyltransferases
virus
insect
Viruses
ecdysteroids
viruses
gene
molting
Insects
Molting
Genes
genes
insects
Larva

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Insecticidal properties of genetically engineered baculoviruses expressing an insect juvenile hormone esterase gene. / Eldridge, R.; O'Reilly, D. R.; Hammock, B. D.; Miller, L. K.

In: Applied and Environmental Microbiology, Vol. 58, No. 5, 1992, p. 1583-1591.

Research output: Contribution to journalArticle

Eldridge, R. ; O'Reilly, D. R. ; Hammock, B. D. ; Miller, L. K. / Insecticidal properties of genetically engineered baculoviruses expressing an insect juvenile hormone esterase gene. In: Applied and Environmental Microbiology. 1992 ; Vol. 58, No. 5. pp. 1583-1591.
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