Abstract
The scorpion Androctonus austrafis has a peptide (AaIT) which selectively targets the insect sodium channel. This mode of action is similar to that of many widely used chemical insecticides. When Bombyx mori larvae were infected with a recombinant baculovirus carrying a synthetic MIT gene, the expressed protein was secreted into the hemolymph and caused symptoms consistent with sodium channel blocking, including tremors and feeding cessation at 40 hr p.i. followed by paralysis and death by 60 hr p.i. Larvae infected with control virus died by 96 hr p.i. These results indicate that foreign genes can be used in recombinant baculoviruses to reduce insect feeding damage and increase the rate of insect kill.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 777-780 |
| Number of pages | 4 |
| Journal | Virology |
| Volume | 184 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1991 |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirus. / Maeda, Susumu; Volrath, Sandra L.; hanzlik, Terry N.; Harper, S. Andrew; Majima, Kei; Maddox, Daryl W.; Hammock, Bruce D.; Fowler, Elizabeth.
In: Virology, Vol. 184, No. 2, 1991, p. 777-780.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Insecticidal effects of an insect-specific neurotoxin expressed by a recombinant baculovirus
AU - Maeda, Susumu
AU - Volrath, Sandra L.
AU - hanzlik, Terry N.
AU - Harper, S. Andrew
AU - Majima, Kei
AU - Maddox, Daryl W.
AU - Hammock, Bruce D.
AU - Fowler, Elizabeth
PY - 1991
Y1 - 1991
N2 - The scorpion Androctonus austrafis has a peptide (AaIT) which selectively targets the insect sodium channel. This mode of action is similar to that of many widely used chemical insecticides. When Bombyx mori larvae were infected with a recombinant baculovirus carrying a synthetic MIT gene, the expressed protein was secreted into the hemolymph and caused symptoms consistent with sodium channel blocking, including tremors and feeding cessation at 40 hr p.i. followed by paralysis and death by 60 hr p.i. Larvae infected with control virus died by 96 hr p.i. These results indicate that foreign genes can be used in recombinant baculoviruses to reduce insect feeding damage and increase the rate of insect kill.
AB - The scorpion Androctonus austrafis has a peptide (AaIT) which selectively targets the insect sodium channel. This mode of action is similar to that of many widely used chemical insecticides. When Bombyx mori larvae were infected with a recombinant baculovirus carrying a synthetic MIT gene, the expressed protein was secreted into the hemolymph and caused symptoms consistent with sodium channel blocking, including tremors and feeding cessation at 40 hr p.i. followed by paralysis and death by 60 hr p.i. Larvae infected with control virus died by 96 hr p.i. These results indicate that foreign genes can be used in recombinant baculoviruses to reduce insect feeding damage and increase the rate of insect kill.
UR - http://www.scopus.com/inward/record.url?scp=0025951015&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025951015&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(91)90451-G
DO - 10.1016/0042-6822(91)90451-G
M3 - Article
VL - 184
SP - 777
EP - 780
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -