Insect cell-expressed p180erbB3 possesses an impaired tyrosine kinase activity

Pamela M. Guy, Jill V. Platko, Lewis C. Cantley, Richard A. Cerione, Kermit L Carraway

Research output: Contribution to journalArticle

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Abstract

Protein kinases share a number of highly conserved or invariant amino acid residues in their catalytic domains, suggesting that these residues are necessary for kinase activity. In p180erbB3, a receptor tyrosine kinase belonging to the epidermal growth factor (EGF) receptor subfamily, three of these residues are altered, suggesting that this protein might have an impaired protein tyrosine kinase activity. To test this hypothesis, we have expressed human EGF receptor and bovine p180erbB3 in insect cells via baculovirus infection and have compared their autophosphorylation and substrate phosphorylation activities. We have found that, while the EGF receptor readily undergoes EGF-stimulated autophosphorylation and catalyzes the incorporation of phosphate into the model substrates (E4Y1)n (random 4:1 copolymer of glutamic acid and tyrosine) and GST-p85 (glutathione S-transferase fusion protein with the 85-kDa subunit of phosphatidylinositol 3-kinase), p180erbB3 autophosphorylation and substrate phosphorylation are at least 2 orders of magnitude less efficient. However, p180erbB3 is capable of binding the ATP analog 5′-p-fluorosulfonylbenzoyladenosine, indicating that the lack of observed kinase activity is probably not due to nonfunctional or denatured receptors expressed by the insect cells. On the basis of these results, we propose that p180erbB3 possesses an impaired intrinsic tyrosine kinase activity.

Original languageEnglish (US)
Pages (from-to)8132-8136
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number17
StatePublished - Aug 16 1994
Externally publishedYes

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Epidermal Growth Factor Receptor
Protein-Tyrosine Kinases
Insects
Phosphotransferases
Phosphorylation
Phosphatidylinositol 3-Kinase
Baculoviridae
Receptor Protein-Tyrosine Kinases
Glutathione Transferase
Epidermal Growth Factor
Protein Kinases
Tyrosine
Glutamic Acid
Catalytic Domain
Proteins
Adenosine Triphosphate
Phosphates
Amino Acids
Infection

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Insect cell-expressed p180erbB3 possesses an impaired tyrosine kinase activity. / Guy, Pamela M.; Platko, Jill V.; Cantley, Lewis C.; Cerione, Richard A.; Carraway, Kermit L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 17, 16.08.1994, p. 8132-8136.

Research output: Contribution to journalArticle

Guy, Pamela M. ; Platko, Jill V. ; Cantley, Lewis C. ; Cerione, Richard A. ; Carraway, Kermit L. / Insect cell-expressed p180erbB3 possesses an impaired tyrosine kinase activity. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 17. pp. 8132-8136.
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