Innate immunity of surfactant proteins A and D in urinary tract infection with uropathogenic Escherichia coli

Fengqi Hu, Guohua Ding, Zhiyong Zhang, Louis A. Gatto, Samuel Hawgood, Francis R Poulain, Robert N. Cooney, Guirong Wang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To investigate the effects of surfactant proteins A and D (SP-A and SP-D, respectively) in urinary tract infection (UTI), SP-A and SP-D double knockout (SP-A/D KO) and wild type (WT) C57BL/6 female mice were infected with uropathogenic Escherichia coli by intravesical inoculation. Compared with WT mice SP-A/D KO mice showed increased susceptibility to UTI, as evidenced by higher bacterial CFU, more infiltrating neutrophils and severe pathological changes. Keratinocyte-derived chemokine increased in the kidney of WT mice but not in SP-A/D KO mice 24 h post-infection. Compared with control, the level of IL-17 was elevated in the kidney of infected WT and SP-A/D KO mice and the level of IL-17 was higher in the infected SP-A/D KO mice than in infected WT mice 24 and 48 h post-infection. The basal level of p38 MAPK phosphorylation in SP-A/D KO mice was higher than in WT mice. The phosphorylated p38 level was elevated in the kidney of WT mice post infection but not in SP-A/D KO mice. Furthermore, in vitro growth of uropathogenic E. coli was inhibited by SP-A and SP-D. We conclude that SP-A and SP-D function as mediators of innate immunity by inhibiting bacterial growth and modulating renal inflammation in part by regulating p38 MAPK-related pathway in murine UTI.

Original languageEnglish (US)
Pages (from-to)9-20
Number of pages12
JournalInnate Immunity
Volume22
Issue number1
DOIs
StatePublished - Jan 1 2016

Fingerprint

Pulmonary Surfactant-Associated Protein D
Uropathogenic Escherichia coli
Pulmonary Surfactant-Associated Protein A
Innate Immunity
Urinary Tract Infections
Kidney
Interleukin-17
p38 Mitogen-Activated Protein Kinases
Infection
Growth

Keywords

  • Innate immunity
  • surfactant protein A
  • surfactant protein D
  • urinary tract infection
  • uropathogenic Escherichia coli

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

Innate immunity of surfactant proteins A and D in urinary tract infection with uropathogenic Escherichia coli. / Hu, Fengqi; Ding, Guohua; Zhang, Zhiyong; Gatto, Louis A.; Hawgood, Samuel; Poulain, Francis R; Cooney, Robert N.; Wang, Guirong.

In: Innate Immunity, Vol. 22, No. 1, 01.01.2016, p. 9-20.

Research output: Contribution to journalArticle

Hu, Fengqi ; Ding, Guohua ; Zhang, Zhiyong ; Gatto, Louis A. ; Hawgood, Samuel ; Poulain, Francis R ; Cooney, Robert N. ; Wang, Guirong. / Innate immunity of surfactant proteins A and D in urinary tract infection with uropathogenic Escherichia coli. In: Innate Immunity. 2016 ; Vol. 22, No. 1. pp. 9-20.
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