Innate immune activation of CD4 T cells in Salmonella-infected mice is dependent on IL-18

Aparna Srinivasan, Rosa Maria Salazar-Gonzalez, Michael Jarcho, Michelle M. Sandau, Leo Lefrancois, Stephen J Mcsorley

Research output: Contribution to journalArticle

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Abstract

Production of IFN-γ by CD4 T cells is generally thought to be mediated by TCR triggering, however, Ag-nonspecific activation of effector CD8 T cells has been reported in infection models. In this study, we demonstrate that Ag-experienced CD4 T cells in the spleen of Salmonella-infected mice acquire the capacity to rapidly secrete IFN-γ in response to stimulation with bacterial lysate or LPS. This innate responsiveness of T cells was transient and most apparent during, and immediately following, active Salmonella infection. Furthermore, innate T cell production of IFN-γ in response to bacterial lysate or LPS was Ag independent and could be induced in Listeria-infected mice and in the absence of MHC class II expression. IL-18 was required for maximal innate responsiveness of CD4 T cells in Salmonella-infected mice and for optimal bacterial clearance in vivo. These data demonstrate that CD4 T cells acquire the capacity to respond to innate stimuli during active bacterial infection, a process that may contribute significantly to amplifying effector responses in vivo.

Original languageEnglish (US)
Pages (from-to)6342-6349
Number of pages8
JournalJournal of Immunology
Volume178
Issue number10
StatePublished - May 15 2007
Externally publishedYes

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Interleukin-18
Salmonella
T-Lymphocytes
Listeria
Salmonella Infections
Bacterial Infections
Spleen
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Srinivasan, A., Salazar-Gonzalez, R. M., Jarcho, M., Sandau, M. M., Lefrancois, L., & Mcsorley, S. J. (2007). Innate immune activation of CD4 T cells in Salmonella-infected mice is dependent on IL-18. Journal of Immunology, 178(10), 6342-6349.

Innate immune activation of CD4 T cells in Salmonella-infected mice is dependent on IL-18. / Srinivasan, Aparna; Salazar-Gonzalez, Rosa Maria; Jarcho, Michael; Sandau, Michelle M.; Lefrancois, Leo; Mcsorley, Stephen J.

In: Journal of Immunology, Vol. 178, No. 10, 15.05.2007, p. 6342-6349.

Research output: Contribution to journalArticle

Srinivasan, A, Salazar-Gonzalez, RM, Jarcho, M, Sandau, MM, Lefrancois, L & Mcsorley, SJ 2007, 'Innate immune activation of CD4 T cells in Salmonella-infected mice is dependent on IL-18', Journal of Immunology, vol. 178, no. 10, pp. 6342-6349.
Srinivasan A, Salazar-Gonzalez RM, Jarcho M, Sandau MM, Lefrancois L, Mcsorley SJ. Innate immune activation of CD4 T cells in Salmonella-infected mice is dependent on IL-18. Journal of Immunology. 2007 May 15;178(10):6342-6349.
Srinivasan, Aparna ; Salazar-Gonzalez, Rosa Maria ; Jarcho, Michael ; Sandau, Michelle M. ; Lefrancois, Leo ; Mcsorley, Stephen J. / Innate immune activation of CD4 T cells in Salmonella-infected mice is dependent on IL-18. In: Journal of Immunology. 2007 ; Vol. 178, No. 10. pp. 6342-6349.
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