Abstract
Background: Murine leukemia virus-type endogenous retroviruses (MuLV-ERVs) constitute ~10% of the mouse genome and are associated with various pathophysiologic processes. In this study, we examined whether MuLV-ERVs' response to burn-elicited stressors is specific for certain lymphocyte populations and/or locations of lymphoid organ.Results: B- and T-cells, which were sorted from nine lymphoid organs of C57BL/6J mice after burn, were subjected to MuLV-ERV expression analyses. Overall, the post-burn MuLV-ERV expression pattern was dependent on lymphocyte type, time after injury, location of lymphoid organ, and MuLV-ERV type. For instance, the MuLV-ERV expression in T-cells from the thymus and three cervical lymph nodes decreased at 3 hours post-burn while the expression of some MuLV-ERVs was augmented in B-cells derived from the mesenteric lymph node. The MuLV-ERV U3 sequences population of the burn-24 hours group was less diverse in comparison to the no burn and burn-3 hours groups. In addition, it was apparent that at the 24 hours time point, the U3 populations of B-cells from both no burn and burn groups were less heterogeneous than the T-cells' U3 populations. Using the U3 sequences, some of which were isolated only from specific experimental groups (B- vs. T-cells; no burn vs. burn), as probes, 51 putative MuLV-ERVs, including 16 full-length proviruses, were mapped followed by characterization of their biologic properties.Conclusion: MuLV-ERVs' response to burn-elicited stressors may be differentially controlled depending on lymphocyte type, location of lymphoid organ, MuLV-ERV type, and stress duration.
| Original language | English (US) |
|---|---|
| Article number | 2 |
| Journal | BMC Immunology |
| Volume | 14 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 5 2013 |
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Keywords
- B-cell
- Burn
- Murine leukemia virus-type endogenous retrovirus
- Stress
- T-cell
ASJC Scopus subject areas
- Immunology
Cite this
Injury-elicited stressors alter endogenous retrovirus expression in lymphocytes depending on cell type and source lymphoid organ. / Lee, Kang Hoon; Lim, Debora; Green, Tajia; Greenhalgh, David G; Cho, Kiho.
In: BMC Immunology, Vol. 14, No. 1, 2, 05.01.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Injury-elicited stressors alter endogenous retrovirus expression in lymphocytes depending on cell type and source lymphoid organ
AU - Lee, Kang Hoon
AU - Lim, Debora
AU - Green, Tajia
AU - Greenhalgh, David G
AU - Cho, Kiho
PY - 2013/1/5
Y1 - 2013/1/5
N2 - Background: Murine leukemia virus-type endogenous retroviruses (MuLV-ERVs) constitute ~10% of the mouse genome and are associated with various pathophysiologic processes. In this study, we examined whether MuLV-ERVs' response to burn-elicited stressors is specific for certain lymphocyte populations and/or locations of lymphoid organ.Results: B- and T-cells, which were sorted from nine lymphoid organs of C57BL/6J mice after burn, were subjected to MuLV-ERV expression analyses. Overall, the post-burn MuLV-ERV expression pattern was dependent on lymphocyte type, time after injury, location of lymphoid organ, and MuLV-ERV type. For instance, the MuLV-ERV expression in T-cells from the thymus and three cervical lymph nodes decreased at 3 hours post-burn while the expression of some MuLV-ERVs was augmented in B-cells derived from the mesenteric lymph node. The MuLV-ERV U3 sequences population of the burn-24 hours group was less diverse in comparison to the no burn and burn-3 hours groups. In addition, it was apparent that at the 24 hours time point, the U3 populations of B-cells from both no burn and burn groups were less heterogeneous than the T-cells' U3 populations. Using the U3 sequences, some of which were isolated only from specific experimental groups (B- vs. T-cells; no burn vs. burn), as probes, 51 putative MuLV-ERVs, including 16 full-length proviruses, were mapped followed by characterization of their biologic properties.Conclusion: MuLV-ERVs' response to burn-elicited stressors may be differentially controlled depending on lymphocyte type, location of lymphoid organ, MuLV-ERV type, and stress duration.
AB - Background: Murine leukemia virus-type endogenous retroviruses (MuLV-ERVs) constitute ~10% of the mouse genome and are associated with various pathophysiologic processes. In this study, we examined whether MuLV-ERVs' response to burn-elicited stressors is specific for certain lymphocyte populations and/or locations of lymphoid organ.Results: B- and T-cells, which were sorted from nine lymphoid organs of C57BL/6J mice after burn, were subjected to MuLV-ERV expression analyses. Overall, the post-burn MuLV-ERV expression pattern was dependent on lymphocyte type, time after injury, location of lymphoid organ, and MuLV-ERV type. For instance, the MuLV-ERV expression in T-cells from the thymus and three cervical lymph nodes decreased at 3 hours post-burn while the expression of some MuLV-ERVs was augmented in B-cells derived from the mesenteric lymph node. The MuLV-ERV U3 sequences population of the burn-24 hours group was less diverse in comparison to the no burn and burn-3 hours groups. In addition, it was apparent that at the 24 hours time point, the U3 populations of B-cells from both no burn and burn groups were less heterogeneous than the T-cells' U3 populations. Using the U3 sequences, some of which were isolated only from specific experimental groups (B- vs. T-cells; no burn vs. burn), as probes, 51 putative MuLV-ERVs, including 16 full-length proviruses, were mapped followed by characterization of their biologic properties.Conclusion: MuLV-ERVs' response to burn-elicited stressors may be differentially controlled depending on lymphocyte type, location of lymphoid organ, MuLV-ERV type, and stress duration.
KW - B-cell
KW - Burn
KW - Murine leukemia virus-type endogenous retrovirus
KW - Stress
KW - T-cell
UR - http://www.scopus.com/inward/record.url?scp=84871790317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871790317&partnerID=8YFLogxK
U2 - 10.1186/1471-2172-14-2
DO - 10.1186/1471-2172-14-2
M3 - Article
VL - 14
JO - BMC Immunology
JF - BMC Immunology
SN - 1471-2172
IS - 1
M1 - 2
ER -