Injectable composite hydrogels for bone repair in a sheep bone defect model

Marissa Gionet-Gonzales, Ganesh C. Ingavle, Charlotte Vorwald, Laurie K. Bohannon, Kaitlin Clark, Larry D Galuppo, Jonathan K Leach

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Effective cell-based therapies for bone healing require the delivery of cells using instructive biomaterials that localize cells at the target site and provide instructional cues. Hydrogels are widely used in such applications, as they are chemically and physically tailorable, but they often lack the desired osteoconductivity of other common materials. To address this shortcoming, we entrapped mesenchymal stem cells (MSCs) in a novel composite hydrogel with enhanced osteoconductivity to promote bone regeneration in an ovine iliac crest bone defect. The composite hydrogel was composed of alginate, chosen for its tailorability, and hyaluronate, selected for its engagement of the CD44 cell receptor present on MSCs. Arginine-Glycine-Aspartic Acid (RGD) was conjugated to the backbone of both polymers to facilitate cell adhesion. Hydrogel osteoconductivity was increased by incorporating biomineralized polymeric microspheres into the network. We hypothesized that the transplantation of autologous ovine MSCs within this composite hydrogel containing mineralized polymeric microspheres would enhance hydrogel osteoconductivity, increase osteogenic potential, and promote bone healing in an ovine bone defect model.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Gionet-Gonzales, M., Ingavle, G. C., Vorwald, C., Bohannon, L. K., Clark, K., Galuppo, L. D., & Leach, J. K. (2019). Injectable composite hydrogels for bone repair in a sheep bone defect model. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.