Abstract
Statement of Purpose: Effective cell-based therapies for bone healing require the delivery of cells using instructive biomaterials that localize cells at the target site and provide instructional cues. Hydrogels are widely used in such applications, as they are chemically and physically tailorable, but they often lack the desired osteoconductivity of other common materials. To address this shortcoming, we entrapped mesenchymal stem cells (MSCs) in a novel composite hydrogel with enhanced osteoconductivity to promote bone regeneration in an ovine iliac crest bone defect. The composite hydrogel was composed of alginate, chosen for its tailorability, and hyaluronate, selected for its engagement of the CD44 cell receptor present on MSCs. Arginine-Glycine-Aspartic Acid (RGD) was conjugated to the backbone of both polymers to facilitate cell adhesion. Hydrogel osteoconductivity was increased by incorporating biomineralized polymeric microspheres into the network. We hypothesized that the transplantation of autologous ovine MSCs within this composite hydrogel containing mineralized polymeric microspheres would enhance hydrogel osteoconductivity, increase osteogenic potential, and promote bone healing in an ovine bone defect model.
| Original language | English (US) |
|---|---|
| Title of host publication | Society for Biomaterials Annual Meeting and Exposition 2019 |
| Subtitle of host publication | The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting |
| Publisher | Society for Biomaterials |
| Number of pages | 1 |
| ISBN (Electronic) | 9781510883901 |
| State | Published - Jan 1 2019 |
| Event | 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States Duration: Apr 3 2019 → Apr 6 2019 |
Publication series
| Name | Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium |
|---|---|
| Volume | 40 |
| ISSN (Print) | 1526-7547 |
Conference
| Conference | 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence |
|---|---|
| Country | United States |
| City | Seattle |
| Period | 4/3/19 → 4/6/19 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Biotechnology
- Biomaterials
- Materials Chemistry
Cite this
Injectable composite hydrogels for bone repair in a sheep bone defect model. / Gionet-Gonzales, Marissa; Ingavle, Ganesh C.; Vorwald, Charlotte; Bohannon, Laurie K.; Clark, Kaitlin; Galuppo, Larry D; Leach, Jonathan K.
Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Injectable composite hydrogels for bone repair in a sheep bone defect model
AU - Gionet-Gonzales, Marissa
AU - Ingavle, Ganesh C.
AU - Vorwald, Charlotte
AU - Bohannon, Laurie K.
AU - Clark, Kaitlin
AU - Galuppo, Larry D
AU - Leach, Jonathan K
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Statement of Purpose: Effective cell-based therapies for bone healing require the delivery of cells using instructive biomaterials that localize cells at the target site and provide instructional cues. Hydrogels are widely used in such applications, as they are chemically and physically tailorable, but they often lack the desired osteoconductivity of other common materials. To address this shortcoming, we entrapped mesenchymal stem cells (MSCs) in a novel composite hydrogel with enhanced osteoconductivity to promote bone regeneration in an ovine iliac crest bone defect. The composite hydrogel was composed of alginate, chosen for its tailorability, and hyaluronate, selected for its engagement of the CD44 cell receptor present on MSCs. Arginine-Glycine-Aspartic Acid (RGD) was conjugated to the backbone of both polymers to facilitate cell adhesion. Hydrogel osteoconductivity was increased by incorporating biomineralized polymeric microspheres into the network. We hypothesized that the transplantation of autologous ovine MSCs within this composite hydrogel containing mineralized polymeric microspheres would enhance hydrogel osteoconductivity, increase osteogenic potential, and promote bone healing in an ovine bone defect model.
AB - Statement of Purpose: Effective cell-based therapies for bone healing require the delivery of cells using instructive biomaterials that localize cells at the target site and provide instructional cues. Hydrogels are widely used in such applications, as they are chemically and physically tailorable, but they often lack the desired osteoconductivity of other common materials. To address this shortcoming, we entrapped mesenchymal stem cells (MSCs) in a novel composite hydrogel with enhanced osteoconductivity to promote bone regeneration in an ovine iliac crest bone defect. The composite hydrogel was composed of alginate, chosen for its tailorability, and hyaluronate, selected for its engagement of the CD44 cell receptor present on MSCs. Arginine-Glycine-Aspartic Acid (RGD) was conjugated to the backbone of both polymers to facilitate cell adhesion. Hydrogel osteoconductivity was increased by incorporating biomineralized polymeric microspheres into the network. We hypothesized that the transplantation of autologous ovine MSCs within this composite hydrogel containing mineralized polymeric microspheres would enhance hydrogel osteoconductivity, increase osteogenic potential, and promote bone healing in an ovine bone defect model.
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M3 - Conference contribution
AN - SCOPUS:85065405699
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
ER -