Inhibitory RNA ligand to reverse transcriptase from feline immunodeficiency virus

Hang Chen, Douglas G. McBroom, Ya Qi Zhu, Larry Gold, Thomas W. North

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

High-affinity, high-specificity RNA ligands for reverse transcriptase from feline immunodeficiency virus (FIV) were isolated from an RNA library by the SELEX (Systematic Evolution of Ligands by Exponential enrichment) procedure. The selected RNA ligands bound to FIV reverse transcriptase with dissociation constants in the nanomolar range. One of the ligands was a potent inhibitor of the RNA-dependent DNA polymerase activity of both the recombinant and the virion-derived FIV reverse transcriptase. It also inhibited the reverse transcriptase from an FIV mutant that is resistant to 3'-azido-3'- deoxythymidine (AZT). The inhibition of FIV reverse transcriptase was competitive with respect to template-primer and noncompetitive with respect to deoxyribonucleoside 5'-triphosphates. This ligand was specific for the FIV enzyme and did not inhibit other reverse transcriptases tested (avian myeloblastosis virus, Moloney murine leukemia virus, and human immunodeficiency virus type I).

Original languageEnglish (US)
Pages (from-to)6923-6930
Number of pages8
JournalBiochemistry
Volume35
Issue number21
DOIs
StatePublished - May 28 1996
Externally publishedYes

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Feline Immunodeficiency Virus
RNA-Directed DNA Polymerase
Viruses
RNA
Ligands
SELEX Aptamer Technique
Avian Myeloblastosis Virus
Deoxyribonucleosides
Moloney murine leukemia virus
Zidovudine
Virion
HIV
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chen, H., McBroom, D. G., Zhu, Y. Q., Gold, L., & North, T. W. (1996). Inhibitory RNA ligand to reverse transcriptase from feline immunodeficiency virus. Biochemistry, 35(21), 6923-6930. https://doi.org/10.1021/bi9600106

Inhibitory RNA ligand to reverse transcriptase from feline immunodeficiency virus. / Chen, Hang; McBroom, Douglas G.; Zhu, Ya Qi; Gold, Larry; North, Thomas W.

In: Biochemistry, Vol. 35, No. 21, 28.05.1996, p. 6923-6930.

Research output: Contribution to journalArticle

Chen, H, McBroom, DG, Zhu, YQ, Gold, L & North, TW 1996, 'Inhibitory RNA ligand to reverse transcriptase from feline immunodeficiency virus', Biochemistry, vol. 35, no. 21, pp. 6923-6930. https://doi.org/10.1021/bi9600106
Chen, Hang ; McBroom, Douglas G. ; Zhu, Ya Qi ; Gold, Larry ; North, Thomas W. / Inhibitory RNA ligand to reverse transcriptase from feline immunodeficiency virus. In: Biochemistry. 1996 ; Vol. 35, No. 21. pp. 6923-6930.
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