Inhibitory properties of nucleic acid-binding ligands on protein synthesis

Abba Malina, Shakila Khan, Coby B. Carlson, Yuri Svitkin, Isabelle Harvey, Nahum Sonenberg, Peter A. Beal, Jerry Pelletier

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The use of small molecule inhibitors in the study of cellular processes is a powerful approach to understanding gene function. During the course of a high throughput screen for novel inhibitors of eukaryotic translation, we identified a number of nucleic acid binding ligands that showed activity in our assay. When tested on a panel of mRNA transcripts displaying different modes of translation initiation, these ligands showed a range of biological activities - with some inhibiting both cap-dependent and internal initiation and others preferentially blocking internal initiation. We used this information to identify a novel threading intercalator that inhibits Hepatitis C virus internal initiation.

Original languageEnglish (US)
Pages (from-to)79-89
Number of pages11
JournalFEBS Letters
Volume579
Issue number1
DOIs
StatePublished - Jan 3 2005
Externally publishedYes

Fingerprint

Nucleic Acids
Intercalating Agents
Ligands
Bioactivity
Viruses
Hepacivirus
Assays
Proteins
Genes
Throughput
Messenger RNA
Molecules

Keywords

  • Hepatitis C virus internal ribosome entry site
  • Nucleic acid intercalator
  • Protein synthesis inhibition
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Malina, A., Khan, S., Carlson, C. B., Svitkin, Y., Harvey, I., Sonenberg, N., ... Pelletier, J. (2005). Inhibitory properties of nucleic acid-binding ligands on protein synthesis. FEBS Letters, 579(1), 79-89. https://doi.org/10.1016/j.febslet.2004.06.103

Inhibitory properties of nucleic acid-binding ligands on protein synthesis. / Malina, Abba; Khan, Shakila; Carlson, Coby B.; Svitkin, Yuri; Harvey, Isabelle; Sonenberg, Nahum; Beal, Peter A.; Pelletier, Jerry.

In: FEBS Letters, Vol. 579, No. 1, 03.01.2005, p. 79-89.

Research output: Contribution to journalArticle

Malina, A, Khan, S, Carlson, CB, Svitkin, Y, Harvey, I, Sonenberg, N, Beal, PA & Pelletier, J 2005, 'Inhibitory properties of nucleic acid-binding ligands on protein synthesis', FEBS Letters, vol. 579, no. 1, pp. 79-89. https://doi.org/10.1016/j.febslet.2004.06.103
Malina A, Khan S, Carlson CB, Svitkin Y, Harvey I, Sonenberg N et al. Inhibitory properties of nucleic acid-binding ligands on protein synthesis. FEBS Letters. 2005 Jan 3;579(1):79-89. https://doi.org/10.1016/j.febslet.2004.06.103
Malina, Abba ; Khan, Shakila ; Carlson, Coby B. ; Svitkin, Yuri ; Harvey, Isabelle ; Sonenberg, Nahum ; Beal, Peter A. ; Pelletier, Jerry. / Inhibitory properties of nucleic acid-binding ligands on protein synthesis. In: FEBS Letters. 2005 ; Vol. 579, No. 1. pp. 79-89.
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