Inhibitory properties of nucleic acid-binding ligands on protein synthesis

Abba Malina, Shakila Khan, Coby B. Carlson, Yuri Svitkin, Isabelle Harvey, Nahum Sonenberg, Peter A. Beal, Jerry Pelletier

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The use of small molecule inhibitors in the study of cellular processes is a powerful approach to understanding gene function. During the course of a high throughput screen for novel inhibitors of eukaryotic translation, we identified a number of nucleic acid binding ligands that showed activity in our assay. When tested on a panel of mRNA transcripts displaying different modes of translation initiation, these ligands showed a range of biological activities - with some inhibiting both cap-dependent and internal initiation and others preferentially blocking internal initiation. We used this information to identify a novel threading intercalator that inhibits Hepatitis C virus internal initiation.

Original languageEnglish (US)
Pages (from-to)79-89
Number of pages11
JournalFEBS Letters
Volume579
Issue number1
DOIs
StatePublished - Jan 3 2005
Externally publishedYes

Keywords

  • Hepatitis C virus internal ribosome entry site
  • Nucleic acid intercalator
  • Protein synthesis inhibition
  • Translation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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