Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells

Suwanna Semsri, Sheryl R. Krig, Lakmal Kotelawala, Colleen A Sweeney, Songyot Anuchapreeda

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate that pure curcumin treatment attenuates WT1 auto-regulation. Interestingly, PKCα inhibition mimicks the repressive effects of pure curcumin in K562 cells. Conversely, myristoylated PKCα over-expression increased WT1 expression and reversed the inhibitory effect of pure curcumin. Our study indicates that pure curcumin attenuates WT1 auto-regulatory function through inhibition of PKCα signaling in K562 cells.

Original languageEnglish (US)
Pages (from-to)2235-2242
Number of pages8
JournalFEBS Letters
Volume585
Issue number14
DOIs
StatePublished - Jul 21 2011

Keywords

  • K562 leukemia cells
  • Protein kinase Cα
  • Pure curcumin
  • WT1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Fingerprint Dive into the research topics of 'Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells'. Together they form a unique fingerprint.

  • Cite this