Inhibitory effects of cocoa flavanols and procyanidin oligomers on free radical-induced erythrocyte hemolysis

Qin Yan Zhu, Roberta R. Holt, Sheryl A. Lazarus, Timothy J. Orozco, Carl L Keen

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Excessive peroxidation of biomembranes is thought to contribute to the initiation and progression of numerous degenerative diseases. The present study examined the inhibitory effects of a cocoa extract, individual cocoa flavanols (-)-epicatechin and (+)-catechin, and procyanidin oligomers (dimer to decamer) isolated from cocoa on rat erythrocyte hemolysis. In vitro, the flavanols and the procyanidin oligomers exhibited dose-dependent protection against 2,2′-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced erythrocyte hemolysis between concentrations of 2.5 and 40 μM. Dimer, trimer, and tetramer showed the strongest inhibitory effects at 10 μM, 59.4%, 66.2%, 70.9%; 20 μM, 84.1%, 87.6%, 81.0%; and 40 μM, 90.2%, 88.9%, 78.6%, respectively. In a subsequent experiment, male Sprague-Dawley rats (∼200 g; n = 5-6) were given a 100-mg intragastric dose of a cocoa extract. Blood was collected over a 4-hr time period. Epicatechin and catechin, and the dimers (-)-epicatechin-(4β>8)-epicatechin (Dimer B2) and (-)-epicatechin-(4>>6)-epicatechin (Dimer B5) were detected in the plasma with concentrations of 6.4 μM, and 217.6, 248.2, and 55.4 nM, respectively. Plasma antioxidant capacity (as measured by the total antioxidant potential [TRAP] assay) was elevated (P < 0.05) between 30 and 240 min following the cocoa extract feeding. Erythrocytes obtained from the cocoa extract-fed animals showed an enhanced resistance to hemolysis (P < 0.05). This enhanced resistance was also observed when erythrocytes from animals fed the cocoa extract were mixed with plasma obtained from animals given water only. Conversely, plasma obtained from rats given the cocoa extract improved the resistance of erythrocytes obtained from rats given water only. These results show cocoa flavanols and procyanidins can provide membrane protective effects.

Original languageEnglish (US)
Pages (from-to)321-329
Number of pages9
JournalExperimental Biology and Medicine
Volume227
Issue number5
StatePublished - May 2002

Fingerprint

Cocoa
Catechin
Hemolysis
Oligomers
Free Radicals
Erythrocytes
Dimers
Rats
Plasmas
Animals
Antioxidants
Proanthocyanidins
Water
procyanidin
Sprague Dawley Rats
Assays
Blood
Membranes

Keywords

  • (+)-catechin
  • (-)-epicatechin
  • (-)-epicatechin- (4β>6)-epicatechin
  • (-)-epicatechin-(4β>8)-epicatechin
  • Cocoa
  • Erythrocyte
  • Flavanol
  • Membrane oxidation
  • Procyanidin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Zhu, Q. Y., Holt, R. R., Lazarus, S. A., Orozco, T. J., & Keen, C. L. (2002). Inhibitory effects of cocoa flavanols and procyanidin oligomers on free radical-induced erythrocyte hemolysis. Experimental Biology and Medicine, 227(5), 321-329.

Inhibitory effects of cocoa flavanols and procyanidin oligomers on free radical-induced erythrocyte hemolysis. / Zhu, Qin Yan; Holt, Roberta R.; Lazarus, Sheryl A.; Orozco, Timothy J.; Keen, Carl L.

In: Experimental Biology and Medicine, Vol. 227, No. 5, 05.2002, p. 321-329.

Research output: Contribution to journalArticle

Zhu, QY, Holt, RR, Lazarus, SA, Orozco, TJ & Keen, CL 2002, 'Inhibitory effects of cocoa flavanols and procyanidin oligomers on free radical-induced erythrocyte hemolysis', Experimental Biology and Medicine, vol. 227, no. 5, pp. 321-329.
Zhu, Qin Yan ; Holt, Roberta R. ; Lazarus, Sheryl A. ; Orozco, Timothy J. ; Keen, Carl L. / Inhibitory effects of cocoa flavanols and procyanidin oligomers on free radical-induced erythrocyte hemolysis. In: Experimental Biology and Medicine. 2002 ; Vol. 227, No. 5. pp. 321-329.
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AU - Keen, Carl L

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N2 - Excessive peroxidation of biomembranes is thought to contribute to the initiation and progression of numerous degenerative diseases. The present study examined the inhibitory effects of a cocoa extract, individual cocoa flavanols (-)-epicatechin and (+)-catechin, and procyanidin oligomers (dimer to decamer) isolated from cocoa on rat erythrocyte hemolysis. In vitro, the flavanols and the procyanidin oligomers exhibited dose-dependent protection against 2,2′-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced erythrocyte hemolysis between concentrations of 2.5 and 40 μM. Dimer, trimer, and tetramer showed the strongest inhibitory effects at 10 μM, 59.4%, 66.2%, 70.9%; 20 μM, 84.1%, 87.6%, 81.0%; and 40 μM, 90.2%, 88.9%, 78.6%, respectively. In a subsequent experiment, male Sprague-Dawley rats (∼200 g; n = 5-6) were given a 100-mg intragastric dose of a cocoa extract. Blood was collected over a 4-hr time period. Epicatechin and catechin, and the dimers (-)-epicatechin-(4β>8)-epicatechin (Dimer B2) and (-)-epicatechin-(4>>6)-epicatechin (Dimer B5) were detected in the plasma with concentrations of 6.4 μM, and 217.6, 248.2, and 55.4 nM, respectively. Plasma antioxidant capacity (as measured by the total antioxidant potential [TRAP] assay) was elevated (P < 0.05) between 30 and 240 min following the cocoa extract feeding. Erythrocytes obtained from the cocoa extract-fed animals showed an enhanced resistance to hemolysis (P < 0.05). This enhanced resistance was also observed when erythrocytes from animals fed the cocoa extract were mixed with plasma obtained from animals given water only. Conversely, plasma obtained from rats given the cocoa extract improved the resistance of erythrocytes obtained from rats given water only. These results show cocoa flavanols and procyanidins can provide membrane protective effects.

AB - Excessive peroxidation of biomembranes is thought to contribute to the initiation and progression of numerous degenerative diseases. The present study examined the inhibitory effects of a cocoa extract, individual cocoa flavanols (-)-epicatechin and (+)-catechin, and procyanidin oligomers (dimer to decamer) isolated from cocoa on rat erythrocyte hemolysis. In vitro, the flavanols and the procyanidin oligomers exhibited dose-dependent protection against 2,2′-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced erythrocyte hemolysis between concentrations of 2.5 and 40 μM. Dimer, trimer, and tetramer showed the strongest inhibitory effects at 10 μM, 59.4%, 66.2%, 70.9%; 20 μM, 84.1%, 87.6%, 81.0%; and 40 μM, 90.2%, 88.9%, 78.6%, respectively. In a subsequent experiment, male Sprague-Dawley rats (∼200 g; n = 5-6) were given a 100-mg intragastric dose of a cocoa extract. Blood was collected over a 4-hr time period. Epicatechin and catechin, and the dimers (-)-epicatechin-(4β>8)-epicatechin (Dimer B2) and (-)-epicatechin-(4>>6)-epicatechin (Dimer B5) were detected in the plasma with concentrations of 6.4 μM, and 217.6, 248.2, and 55.4 nM, respectively. Plasma antioxidant capacity (as measured by the total antioxidant potential [TRAP] assay) was elevated (P < 0.05) between 30 and 240 min following the cocoa extract feeding. Erythrocytes obtained from the cocoa extract-fed animals showed an enhanced resistance to hemolysis (P < 0.05). This enhanced resistance was also observed when erythrocytes from animals fed the cocoa extract were mixed with plasma obtained from animals given water only. Conversely, plasma obtained from rats given the cocoa extract improved the resistance of erythrocytes obtained from rats given water only. These results show cocoa flavanols and procyanidins can provide membrane protective effects.

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KW - (-)-epicatechin-(4β>8)-epicatechin

KW - Cocoa

KW - Erythrocyte

KW - Flavanol

KW - Membrane oxidation

KW - Procyanidin

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