Inhibitory effect of paeoniflorin on rat fibroblast-like synoviocytes hyperplasia induced by PGE2 via β-arrestin 2 regulating EP2 receptor

Qingtong Wang, Yu Kun Ma, Bei Huang, Wei Wei

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Aim: To investigate the potential molecular mechanism of paeoniflorin (Pae) in inhibiting PGE2 induced rat fibroblast-like synoviocytes (FLSs) hyperplasy. Methods: Rat FLSs were cultured in the presence of prostaglandin E2 (PGE2) with or without different concentrations of Pae. FLSs proliferations were determined by 3H-TdR incorporation assay. Cyclic adenosine monophosphate (cAMP) levels in synoviocytes were assessed by radioimmunoassay (RIA). The expressions of EP2 and β-arrestin 2 in whole FLSs or on membrane were measured by Western blot or flow cytometry. Results: Pae significantly inhibited rat FLS proliferation induced by PGE2 via increasing cAMP levels. High levels of total EP2, β-arrestin 2 and membrane β-arrestin 2 expression in FLSs induced by PGE2 were decreased by different concentrations of Pae administration. EP2 was downregulated on FLSs membrane after PGE2 stimulation; however, was restored by Pae in various degrees. Conclusions: Our findings suggest that Pae inhibits rat FLSs proliferation induced by PGE2 via arresting EP2 receptor internalization by inhibiting β-arrestin 2 recruit to cell membrane.

Original languageEnglish (US)
Pages (from-to)43-47
Number of pages5
JournalChinese Pharmacological Bulletin
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Keywords

  • EP
  • Fibroblast-like synoviocytes
  • Paeoniflorin
  • Rheumatoid arthritis
  • β-arrestin

ASJC Scopus subject areas

  • Pharmacology

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