Inhibitory effect of cholesterol oxides on low density lipoprotein receptor gene expression

Shi Kaung Peng, Xiaoyan Zhang, Ning Ning Chai, Yu-Jui Yvonne Wan, Robert J. Morin

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The effects of cholesterol oxides on low density lipoprotein receptor (LDLR) gene expression were investigated. Cultured rabbit aortic smooth muscle cells were incubated with 1, 2, and 5 μg/ml culture medium concentrations of pure cholesterol, 25-hydroxycholesterol (25-OH), 7- ketocholesterol (7-keto), cholestane-3β, 5α, 6β-triol (triol) and cholesterol-5α, 6α-epoxide (epoxide) for 12 hours and with vehicle only as control. Total mRNAs were extracted and electrophoresed. Northern blot hybridization analyses were performed. The results showed mRNA expressions of LDLR gene were inhibited to 16.1 ± 4.4%, 33.8 ± 0.6%, 42.8 ± 1.8% and 46.9 ± 3.9% of control by 25-OH, 7-keto, epoxide and triol respectively. Pure cholesterol showed only minimal inhibition. The inhibitions were time dependent. Although cholesterol oxides have been shown to alter many membrane-related functions and the LDLR domain are located in the cell membrane. The findings of this study suggested that the cholesterol oxides exerted their repressive actions on LDLR function primarily by down- regulating LDLR gene expression rather than directly upon cell membrane.

Original languageEnglish (US)
Pages (from-to)61-79
Number of pages19
JournalArtery
Volume22
Issue number2
StatePublished - 1996

Keywords

  • arterial smooth muscle cell
  • atherosclerosis
  • Cholesterol oxides
  • gene expression
  • LDL receptors

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Peng, S. K., Zhang, X., Chai, N. N., Wan, Y-J. Y., & Morin, R. J. (1996). Inhibitory effect of cholesterol oxides on low density lipoprotein receptor gene expression. Artery, 22(2), 61-79.