Inhibition of tobacco smoke-induced lung inflammation by a catalytic antioxidant

Kevin R. Smith, Dale L. Uyeminami, Urmila P. Kodavanti, James D. Crapo, Ling Yi Chang, Kent E Pinkerton

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Cigarette smokers experience airway inflammation and epithelial damage, the mechanisms of which are unknown. One potential cause may be free radicals either in tobacco smoke or produced during persistent inflammation. Inflammation may also be a driving force to cause airway epithelium to undergo changes leading to squamous cell metaplasia. To test whether tobacco smoke-induced inflammation could be reduced by a catalytic antioxidant, manganese(III)meso-tetrakis(N,N′-diethyl-1,3-imidazolium-2-yl) porphyrin (AEOL 10150) was given by intratracheal instillation to rats exposed to filtered air or tobacco smoke. Exposure to tobacco smoke for 2 d or 8 weeks (6 h/d, 3 d/week) significantly increased the number of cells recovered by bronchoalveolar lavage (BAL). AEOL 10150 significantly decreased BAL cell number in tobacco smoke-treated rats. Significant reductions in neutrophils were noted at 2 d and macrophages at 8 weeks. Lymphocytes were significantly reduced by AEOL 10150 at both time points. Squamous cell metaplasia following 8 weeks of tobacco smoke exposure was 12% of the total airway epithelial area in animals exposed to tobacco smoke without AEOL 10150, compared with 2% in animals exposed to tobacco smoke, but treated with AEOL 10150 (p < .05). We conclude that a synthetic catalytic antioxidant decreased the adverse effects of exposure to tobacco smoke.

Original languageEnglish (US)
Pages (from-to)1106-1114
Number of pages9
JournalFree Radical Biology and Medicine
Volume33
Issue number8
DOIs
StatePublished - Oct 15 2002

Fingerprint

Tobacco
Smoke
Pneumonia
Antioxidants
Inflammation
Metaplasia
Bronchoalveolar Lavage
Rats
Animals
Cell Count
Epithelial Cells
Lymphocytes
Macrophages
Porphyrins
Manganese
Tobacco Products
Free Radicals
Neutrophils
Epithelium
Air

Keywords

  • AEOL 10150
  • Catalytic antioxidant
  • Free radicals
  • Inflammation
  • Metalloporphyrins
  • Squamous cell metaplasia
  • Tobacco smoke

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Inhibition of tobacco smoke-induced lung inflammation by a catalytic antioxidant. / Smith, Kevin R.; Uyeminami, Dale L.; Kodavanti, Urmila P.; Crapo, James D.; Chang, Ling Yi; Pinkerton, Kent E.

In: Free Radical Biology and Medicine, Vol. 33, No. 8, 15.10.2002, p. 1106-1114.

Research output: Contribution to journalArticle

Smith, Kevin R. ; Uyeminami, Dale L. ; Kodavanti, Urmila P. ; Crapo, James D. ; Chang, Ling Yi ; Pinkerton, Kent E. / Inhibition of tobacco smoke-induced lung inflammation by a catalytic antioxidant. In: Free Radical Biology and Medicine. 2002 ; Vol. 33, No. 8. pp. 1106-1114.
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