Inhibition of Src family kinases protects hippocampal neurons and improves cognitive function after traumatic brain injury

Da Liu, Frank R Sharp, Ken C. Van, Bradley Ander, Rahil Ghiasvand, Xinhua Zhan, Boryana Stamova, Glen C. Jickling, Bruce G Lyeth

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Traumatic brain injury (TBI) is often associated with intracerebral and intraventricular hemorrhage. Thrombin is a neurotoxin generated at bleeding sites fater TBI and can lead to cell death and subsequent cognitive dysfunction via activation of Src family kinases (SFKs). We hypothesize that inhibiting SFKs can protect hippocampal neurons and improve cognitive memory function after TBI. To test these hypotheses, we show that moderate lateral fluid percussion (LFP) TBI in adult rats produces bleeding into the cerebrospinal fluid (CSF) in both lateral ventricles, which elevates oxyhemoglobin and thrombin levels in the CSF, activates the SFK family member Fyn, and increases Rho-kinase 1(ROCK1) expression. Systemic administration of the SFK inhibitor, PP2, immediately after moderate TBI blocks ROCK1 expression, protects hippocampal CA2/3 neurons, and improves spatial memory function. These data suggest the possibility that inhibiting SFKs after TBI might improve clinical outcomes.

Original languageEnglish (US)
Pages (from-to)1268-1276
Number of pages9
JournalJournal of Neurotrauma
Volume31
Issue number14
DOIs
StatePublished - Jul 15 2014

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Keywords

  • Cognitive memory deficits
  • Hemorrhage
  • Src family kinases (SFKs)
  • Thrombin
  • Traumatic brain injury (TBI)

ASJC Scopus subject areas

  • Clinical Neurology
  • Medicine(all)

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