Inhibition of proliferation and induction of apoptosis in SNU-1 human gastric cancer cells by the plant sulfolipid, sulfoquinovosyldiacylglycerol

Mary E. Quasney, Lynne C. Carter, Carol Oxford, Steven M. Watkins, M. Eric Gershwin, J. Bruce German

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The sulfoglycolipid sulfoquinovosyldiacylglycerol is present in the membranes of photosynthetic organisms. This sulfolipid reportedly has pharmaceutical potential as an antiviral and antitumor agent, although no studies have examined these properties of the sulfolipids that are consumed in plant foods. This study examined the biological effects of sulfoquinovosyldiacylglycerol on the human gastric cancer cell line SNU-1. SNU-1 cells were grown in the absence and of presence of 1 μM, 100 μM or 1 mM sulfoquinovosyldiacylglycerol for up to 72 hours. Cell proliferation and viability were determined. The cells were analyzed for nuclear morphological changes by fluorescence microscopy and for DNAase-mediated DNA cleavage by flow cytometry and TUNEL detection. As indicated by cell number, the proliferation of SNU-1 cells by 72 hours of culture in the presence of 100 μM and 1 mM SQDG was inhibited 24 and 100%, respectively, as compared with the number of SNU-1 cells cultured in the absence of SQDG. Inhibition of cell proliferation by 100 μM sulfoquinovosyldiacylglycerol was in part associated with apoptotic cell death, as shown by changes in nuclear morphology and DNA fragmentation, whereas incubation of cells with 1 mM sulfoquinovosyldiacylglycerol caused necrotic cell death. Treatment of SNU-1 cells with sulfoquinovosyldiacylglycerol did not result in cell cycle arrest. The antiproliferative and apoptotic effects of sulfoquinovosyldiacylglycerol on SNU-1 gastric cancer cells revealed in this study suggest that this common dietary sulfolipid has intriguing potential as a chemopreventive or chemotherapeutic agent.

Original languageEnglish (US)
Pages (from-to)310-315
Number of pages6
JournalJournal of Nutritional Biochemistry
Volume12
Issue number5
DOIs
StatePublished - 2001

Fingerprint

Plant Cells
Stomach Neoplasms
Cells
Apoptosis
Cell proliferation
Cell death
Cell Proliferation
Deoxyribonucleases
Flow cytometry
Fluorescence microscopy
DNA
Cell Death
Antineoplastic Agents
Antiviral Agents
Edible Plants
DNA Cleavage
In Situ Nick-End Labeling
DNA Fragmentation
Cell Cycle Checkpoints
Membranes

Keywords

  • Apoptosis
  • Cell proliferation
  • SNU-1 cancer cells
  • Sulfolipid
  • Sulfoquinovosyldiacylglycerol

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Inhibition of proliferation and induction of apoptosis in SNU-1 human gastric cancer cells by the plant sulfolipid, sulfoquinovosyldiacylglycerol. / Quasney, Mary E.; Carter, Lynne C.; Oxford, Carol; Watkins, Steven M.; Gershwin, M. Eric; German, J. Bruce.

In: Journal of Nutritional Biochemistry, Vol. 12, No. 5, 2001, p. 310-315.

Research output: Contribution to journalArticle

Quasney, Mary E. ; Carter, Lynne C. ; Oxford, Carol ; Watkins, Steven M. ; Gershwin, M. Eric ; German, J. Bruce. / Inhibition of proliferation and induction of apoptosis in SNU-1 human gastric cancer cells by the plant sulfolipid, sulfoquinovosyldiacylglycerol. In: Journal of Nutritional Biochemistry. 2001 ; Vol. 12, No. 5. pp. 310-315.
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AU - Gershwin, M. Eric

AU - German, J. Bruce

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