Inhibition of perilipin 2 expression reduces pro-inflammatory gene expression and increases lipid droplet size

Jennifer E. Norman, Hnin H. Aung, Dennis W Wilson, John C Rutledge

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Our lab previously demonstrated that triglyceride-rich lipoprotein (TGRL) lipolysis products induce lipid droplet formation and pro-inflammatory gene expression in monocytes. We hypothesized that the inhibition of perilipin 2 expression in THP-1 monocytes would reduce lipid droplet formation and suppress pro-inflammatory gene expression induced by TGRL lipolysis products. In the current study, we use microarray analysis to identify gene expression altered by TGRL lipolysis products in THP-1 monocytes. We confirmed the expression of selected genes by quantitative reverse transcription PCR and characterized lipid droplet formation in these cells after exposure to TGRL lipolysis products. Using siRNA inhibition of perilipin 2 expression, we examined the role of perilipin 2 in the response of THP-1 monocytes to TGRL lipolysis products. We found that perilipin 2 siRNA increased the intracellular triglyceride content, increased the size of lipid droplets, and reduced pro-atherogenic and pro-inflammatory gene expression. We saw a reduction of serum/glucocorticoid kinase 1, v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian), chemokine (C-C motif) ligand 3, and interleukin 8 gene expression induced by TGRL lipolysis products. This study supports previous findings that reduction of perilipin 2 expression is protective against atherogenesis, while finding an unexpected increase in lipid droplet size with reduced perilipin 2 expression.

Original languageEnglish (US)
Pages (from-to)6245-6256
Number of pages12
JournalFood & function
Volume9
Issue number12
DOIs
StatePublished - Dec 13 2018

Fingerprint

adipophilin
droplet size
Lipolysis
lipolysis
lipoproteins
Triglycerides
Lipoproteins
triacylglycerols
Gene Expression
gene expression
monocytes
droplets
lipids
Monocytes
small interfering RNA
Small Interfering RNA
Chemokine CCL8
Chemokine CCL3
atherogenesis
fibrosarcoma

ASJC Scopus subject areas

  • Food Science

Cite this

Inhibition of perilipin 2 expression reduces pro-inflammatory gene expression and increases lipid droplet size. / Norman, Jennifer E.; Aung, Hnin H.; Wilson, Dennis W; Rutledge, John C.

In: Food & function, Vol. 9, No. 12, 13.12.2018, p. 6245-6256.

Research output: Contribution to journalArticle

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