Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids

Ming Wen Li, Ashley I. Yudin, Catherine A. VandeVoort, Khalida Sabeur, Paul Primakoff, James W. Overstreet

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of 50-200 μM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin, and apigenin at 100 μM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.

Original languageEnglish (US)
Pages (from-to)1383-1389
Number of pages7
JournalBiology of Reproduction
Volume56
Issue number6
DOIs
StatePublished - Jun 1997

Fingerprint

Hyaluronoglucosaminidase
Flavonoids
Haplorhini
Spermatozoa
Apigenin
Tannins
Quercetin
Sperm Motility
Cricetinae
Sperm-Ovum Interactions
Acrosome Reaction
Macaca fascicularis
Fertilization
kaempferol

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Li, M. W., Yudin, A. I., VandeVoort, C. A., Sabeur, K., Primakoff, P., & Overstreet, J. W. (1997). Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids. Biology of Reproduction, 56(6), 1383-1389. https://doi.org/10.1095/biolreprod56.6.1383

Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids. / Li, Ming Wen; Yudin, Ashley I.; VandeVoort, Catherine A.; Sabeur, Khalida; Primakoff, Paul; Overstreet, James W.

In: Biology of Reproduction, Vol. 56, No. 6, 06.1997, p. 1383-1389.

Research output: Contribution to journalArticle

Li, MW, Yudin, AI, VandeVoort, CA, Sabeur, K, Primakoff, P & Overstreet, JW 1997, 'Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids', Biology of Reproduction, vol. 56, no. 6, pp. 1383-1389. https://doi.org/10.1095/biolreprod56.6.1383
Li, Ming Wen ; Yudin, Ashley I. ; VandeVoort, Catherine A. ; Sabeur, Khalida ; Primakoff, Paul ; Overstreet, James W. / Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids. In: Biology of Reproduction. 1997 ; Vol. 56, No. 6. pp. 1383-1389.
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