Abstract
A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of 50-200 μM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin, and apigenin at 100 μM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1383-1389 |
| Number of pages | 7 |
| Journal | Biology of Reproduction |
| Volume | 56 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1997 |
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ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Embryology
Cite this
Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids. / Li, Ming Wen; Yudin, Ashley I.; VandeVoort, Catherine A.; Sabeur, Khalida; Primakoff, Paul; Overstreet, James W.
In: Biology of Reproduction, Vol. 56, No. 6, 06.1997, p. 1383-1389.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids
AU - Li, Ming Wen
AU - Yudin, Ashley I.
AU - VandeVoort, Catherine A.
AU - Sabeur, Khalida
AU - Primakoff, Paul
AU - Overstreet, James W.
PY - 1997/6
Y1 - 1997/6
N2 - A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of 50-200 μM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin, and apigenin at 100 μM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.
AB - A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of 50-200 μM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin, and apigenin at 100 μM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.
UR - http://www.scopus.com/inward/record.url?scp=0030947633&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030947633&partnerID=8YFLogxK
U2 - 10.1095/biolreprod56.6.1383
DO - 10.1095/biolreprod56.6.1383
M3 - Article
C2 - 9166689
AN - SCOPUS:0030947633
VL - 56
SP - 1383
EP - 1389
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 6
ER -