Inhibition of human and murine cytosolic epoxide hydrolase by group-selective reagents

Eric C. Dietze, Joseph Stephens, Jacques Magdalou, Daniel M. Bender, Mary Moyer, Beth Fowler, Bruce D. Hammock

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. 1. Human and murine cytosolic epoxide hydrolase were inhibited by thiol-, imidazole- and carboxyl-selective reagents. They were not inhibited by amino-, guanido- or activated serine-selective reagents. 2. 2. Murine, but not human, cytosolic epoxide hydrolase was inhibited by N-bromosuccinimide, a tryptophan selective reagent. 3. 3. Based on sequence data from peptides isolated from CNBr digests, human and murine CEH share areas of sequence homology. Of the five unique human CEH CNBr peptides sequenced, three shared common sequences with one of the unique murine CEH CNBr peptides. The human and murine CEH peptides with common sequences had between 64 and 78% sequence identity. 4. 4. A cysteine important for the activity of murine CEH appears not to be in the active site as judged by N-phenylmaleimide inhibition in the presence and absence of either (2S,3S)-2,3-epoxy-3-(4-nitrophenyl)glycidol, a competitive inhibitor, or trans-stilbene oxide, a substrate.

Original languageEnglish (US)
Pages (from-to)299-308
Number of pages10
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume104
Issue number2
DOIs
StatePublished - 1993

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Epoxide Hydrolases
Peptides
glycidol
Bromosuccinimide
Sulfhydryl Compounds
Tryptophan
Serine
Cysteine
Sequence Homology
Catalytic Domain
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this

Inhibition of human and murine cytosolic epoxide hydrolase by group-selective reagents. / Dietze, Eric C.; Stephens, Joseph; Magdalou, Jacques; Bender, Daniel M.; Moyer, Mary; Fowler, Beth; Hammock, Bruce D.

In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and, Vol. 104, No. 2, 1993, p. 299-308.

Research output: Contribution to journalArticle

Dietze, Eric C. ; Stephens, Joseph ; Magdalou, Jacques ; Bender, Daniel M. ; Moyer, Mary ; Fowler, Beth ; Hammock, Bruce D. / Inhibition of human and murine cytosolic epoxide hydrolase by group-selective reagents. In: Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 1993 ; Vol. 104, No. 2. pp. 299-308.
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AU - Hammock, Bruce D.

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AB - 1. 1. Human and murine cytosolic epoxide hydrolase were inhibited by thiol-, imidazole- and carboxyl-selective reagents. They were not inhibited by amino-, guanido- or activated serine-selective reagents. 2. 2. Murine, but not human, cytosolic epoxide hydrolase was inhibited by N-bromosuccinimide, a tryptophan selective reagent. 3. 3. Based on sequence data from peptides isolated from CNBr digests, human and murine CEH share areas of sequence homology. Of the five unique human CEH CNBr peptides sequenced, three shared common sequences with one of the unique murine CEH CNBr peptides. The human and murine CEH peptides with common sequences had between 64 and 78% sequence identity. 4. 4. A cysteine important for the activity of murine CEH appears not to be in the active site as judged by N-phenylmaleimide inhibition in the presence and absence of either (2S,3S)-2,3-epoxy-3-(4-nitrophenyl)glycidol, a competitive inhibitor, or trans-stilbene oxide, a substrate.

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