Inhibition of HIV-1 integration in ex vivo-infected CD4 T cells from elite controllers

Maria J. Buzon, Katherine Seiss, Robert H Weiss, Abraham L. Brass, Eric S. Rosenberg, Florencia Pereyra, Xu G. Yu, Mathias Lichterfeld

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Elite controllers spontaneously maintain undetectable levels of HIV-1 replication for reasons that remain unclear. Here, we show that in elite controllers, direct ex vivo infection of purified CD4 T cells without prior in vitro activation results in disproportionately low levels of integrated HIV-1 DNA relative to the quantity of reverse transcripts, while the levels of two-long terminal repeat (2-LTR) circles were excessively elevated relative to those of integrated HIV-1 DNA. This indicates that chromosomal HIV-1 integration is inhibited in ex vivo-infected CD4 T cells from elite controllers. This defect in HIV-1 integration was unrelated to p21, a host protein that can restrict early HIV-1 replication steps, and was not visible following infection of in vitroactivated CD4 T cells from elite controllers. These data contribute to increasing evidence that intrinsic inhibition of specific HIV-1 replication steps plays an important role in the ability of elite controllers to maintain undetectable viral loads.

Original languageEnglish (US)
Pages (from-to)9646-9650
Number of pages5
JournalJournal of Virology
Volume85
Issue number18
DOIs
StatePublished - Sep 2011

ASJC Scopus subject areas

  • Immunology
  • Virology

Fingerprint Dive into the research topics of 'Inhibition of HIV-1 integration in ex vivo-infected CD4 T cells from elite controllers'. Together they form a unique fingerprint.

  • Cite this

    Buzon, M. J., Seiss, K., Weiss, R. H., Brass, A. L., Rosenberg, E. S., Pereyra, F., Yu, X. G., & Lichterfeld, M. (2011). Inhibition of HIV-1 integration in ex vivo-infected CD4 T cells from elite controllers. Journal of Virology, 85(18), 9646-9650. https://doi.org/10.1128/JVI.05327-11